Department of Biomaterials and Medical Devices, Faculty of Medical Engineering, Politehnica University of Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, Romania.
Institute for Research on the Quality of Society and the Sciences of Education, University Constantin Brancusi of Targu Jiu, Republicii 1, 210185 Targu Jiu, Romania.
Int J Mol Sci. 2023 Sep 29;24(19):14722. doi: 10.3390/ijms241914722.
Cellular asymmetry is an important element of efficiency in the compartmentalization of intracellular chemical reactions that ensure efficient tissue function. Improving the current 3D printing methods by using cellular asymmetry is essential in producing complex tissues and organs such as the liver. The use of cell spots containing at least two cells and basement membrane-like bio support materials allows cells to be tethered at two points on the basement membrane and with another cell in order to maintain cell asymmetry. Our model is a new type of 3D bioprinter that uses oriented multicellular complexes with cellular asymmetry. This novel approach is necessary to replace the sequential and slow processes of organogenesis with rapid methods of growth and 3D organ printing. The use of the extracellular matrix in the process of bioprinting with cells allows one to preserve the cellular asymmetry in the 3D printing process and thus preserve the compartmentalization of biological processes and metabolic efficiency.
细胞不对称性是细胞内化学反应分隔的效率的重要因素，这确保了组织功能的高效。通过利用细胞不对称性改进当前的 3D 打印方法对于生产复杂的组织和器官（如肝脏）至关重要。使用包含至少两个细胞的细胞点和基底膜样生物支持材料，可以将细胞固定在基底膜上的两个点上，并与另一个细胞相连，以保持细胞的不对称性。我们的模型是一种新型的 3D 生物打印机，它使用具有细胞不对称性的定向多细胞复合物。这种新方法对于用快速的生长和 3D 器官打印方法取代器官发生的顺序和缓慢的过程是必要的。在细胞生物打印过程中使用细胞外基质可以在 3D 打印过程中保持细胞的不对称性，从而保持生物过程的分隔和代谢效率。
