芍药苷对胃癌细胞中Hippo信号通路的抗肿瘤作用
Antitumor Effects of Paeoniflorin on Hippo Signaling Pathway in Gastric Cancer Cells.
作者信息
Niu Kai, Liu Yanling, Zhou Zijun, Wu Xuefeng, Wang Huaiwu, Yan Jingzhe
机构信息
Department of Thoracic Oncology, Jilin Province Cancer Hospital, Changchun, Jilin 130012, China.
Department of Internal Medicine, Jilin Province Cancer Hospital, Changchun, Jilin 130012, China.
出版信息
J Oncol. 2021 Jan 19;2021:4724938. doi: 10.1155/2021/4724938. eCollection 2021.
BACKGROUND
Paeoniflorin has been reported to exert antitumor effects on human cancers. However, the role of paeoniflorin in gastric cancer and the underlying molecular mechanism are unelucidated. Therefore, we determined whether paeoniflorin could exhibit anticancer activity in gastric cancer cells.
METHODS
MTT was used to measure the viability of cells after paeoniflorin treatment. FACS was performed to examine cell apoptosis. Wound healing and transwell invasion assays were conducted to examine cell migratory and invasive activities. Western blotting was used to explore the mechanism by which paeoniflorin exerted tumor suppressive effects.
RESULTS
We found that paeoniflorin suppressed cell growth, enhanced apoptosis, and reduced cell invasion. Notably, we showed that paeoniflorin inhibited the expression of TAZ in gastric cancer cells. The overexpression of TAZ abrogated the antitumor activity of paeoniflorin in gastric cancer cells. In contrast, the downregulation of TAZ promoted the tumor suppressive effects of paeoniflorin treatment.
CONCLUSION
Hence, targeting TAZ with paeoniflorin could be a novel approach for the treatment of human gastric cancer.
背景
据报道，芍药苷对人类癌症具有抗肿瘤作用。然而，芍药苷在胃癌中的作用及其潜在的分子机制尚不清楚。因此，我们确定了芍药苷是否能在胃癌细胞中表现出抗癌活性。
方法
采用MTT法检测芍药苷处理后细胞的活力。进行流式细胞术检测细胞凋亡。进行伤口愈合和Transwell侵袭实验检测细胞的迁移和侵袭活性。采用蛋白质免疫印迹法探讨芍药苷发挥肿瘤抑制作用的机制。
结果
我们发现芍药苷抑制细胞生长，增强细胞凋亡，并减少细胞侵袭。值得注意的是，我们发现芍药苷抑制胃癌细胞中TAZ的表达。TAZ的过表达消除了芍药苷在胃癌细胞中的抗肿瘤活性。相反，TAZ的下调增强了芍药苷处理的肿瘤抑制作用。
结论
因此，以TAZ为靶点使用芍药苷可能是治疗人类胃癌的一种新方法。
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