Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany.
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany.
Transbound Emerg Dis. 2022 Mar;69(2):886-890. doi: 10.1111/tbed.14015. Epub 2021 Feb 21.
The striped field mouse (Apodemus agrarius) is known to carry several zoonotic pathogens, including Leptospira spp. and Dobrava-Belgrade orthohantavirus (DOBV). Since its first detection in 1996 in south-east Austria, the striped field mouse has further expanded its range in Austria. Here, we screened 35 striped field mice collected in an Austrian region near the Hungarian border for DOBV, Leptospira spp. and seven vector-borne pathogens. Hantavirus RT-PCR screening and DOBV IgG ELISA analysis led to the detection of two DOBV-positive striped field mice. The complete coding sequences of all three genome segments of both strains were determined by a combination of target enrichment and next-generation sequencing. Both complete coding S segment sequences clustered within the DOBV genotype Kurkino clade with the highest similarity to a sequence from Hungary. In one of 35 striped field mice, Leptospira borgpetersenii sequence type (ST) 146 was detected. Bartonella spp., Borrelia miyamotoi and Neoehrlichia mikurensis DNA was detected in four, one and two of 32 mice, respectively. Babesia, Anaplasma, Ehrlichia and Rickettsia specific DNA was not detected. Future investigations will have to determine the prevalence and invasion of these pathogens with the ongoing range expansion of the striped field mouse in Austria.
横纹田鼠已知携带几种人畜共患病原体，包括钩端螺旋体属和多布拉伐-贝尔格莱德正呼肠孤病毒（DOBV）。自 1996 年首次在奥地利东南部发现以来，横纹田鼠在奥地利的分布范围进一步扩大。在这里，我们对在奥地利与匈牙利边境附近一个地区采集的 35 只横纹田鼠进行了 DOBV、钩端螺旋体属和七种媒介传播病原体的筛查。汉坦病毒 RT-PCR 筛查和 DOBV IgG ELISA 分析导致检测到两只 DOBV 阳性的横纹田鼠。通过目标富集和下一代测序相结合的方法，确定了这两个毒株所有三个基因组片段的完整编码序列。两个完整的编码 S 片段序列都聚类在 DOBV 基因型 Kurkino 分支内，与来自匈牙利的序列具有最高的相似性。在 35 只横纹田鼠中的一只中，检测到了博氏包柔螺旋体 146 型。在 32 只老鼠中的 4 只、1 只和 2 只中分别检测到了巴尔通体属、米亚马托博氏螺旋体和新立克次体。未检测到巴贝斯虫、无形体、埃立克体和立克次体的特异性 DNA。未来的研究将不得不确定这些病原体的流行和入侵情况，因为横纹田鼠在奥地利的分布范围正在不断扩大。
