Department of Pediatrics, Hsinchu Mackay Memorial Hospital, Hsinchu City, 300, Taiwan.
Department of Medicine, Mackay Medical College, New Taipei City, 252, Taiwan.
Sci Rep. 2021 Feb 3;11(1):2856. doi: 10.1038/s41598-021-82584-8.
Early childhood is a critical stage for the foundation and development of the gut microbiome, large amounts of essential nutrients are required such as vitamin D. Vitamin D plays an important role in regulating calcium homeostasis, and deficiency can impair bone mineralization. In addition, most people know that breastfeeding is advocated to be the best thing for a newborn; however, exclusively breastfeeding infants are not easily able to absorb an adequate amount of vitamin D from breast milk. Understanding the effects of vitamin D supplementation on gut microbiome can improve the knowledge of infant health and development. A total of 62 fecal sample from healthy infants were collected in Taiwan. Of the 62 infants, 31 were exclusively breastfed infants and 31 were mixed- or formula-fed infants. For each feeding type, one subgroup of infants received 400 IU of vitamin D per day, and the remaining infants received a placebo. In total, there are 15 breastfed and 20 formula-fed infants with additional vitamin D supplementation, and 16 breastfed and 11 formula-fed infants belong to control group, respectively. We performed a comparative metagenomic analysis to investigate the distribution and diversity of infant gut microbiota among different types of feeding regimes with and without vitamin D supplementation. Our results reveal that the characteristics of infant gut microbiota not only depend on the feeding types but also on nutrients intake, and demonstrated that the vitamin D plays an important role in modulating the infant gut microbiota, especially increase the proportion of probiotics in breast-fed infants.
儿童早期是肠道微生物组形成和发展的关键阶段，需要大量的必需营养素，如维生素 D。维生素 D 在调节钙稳态方面发挥着重要作用，缺乏会损害骨骼矿化。此外，大多数人都知道提倡母乳喂养是新生儿最好的选择；然而，纯母乳喂养的婴儿不容易从母乳中吸收足够的维生素 D。了解维生素 D 补充对肠道微生物组的影响，可以提高人们对婴儿健康和发育的认识。在台湾，共收集了 62 名健康婴儿的粪便样本。在这 62 名婴儿中，31 名是纯母乳喂养的婴儿，31 名是混合喂养或配方奶喂养的婴儿。对于每种喂养方式，一组婴儿每天接受 400IU 的维生素 D，其余婴儿接受安慰剂。共有 15 名母乳喂养且额外补充维生素 D 的婴儿和 20 名配方奶喂养且额外补充维生素 D 的婴儿，以及 16 名母乳喂养且属于对照组的婴儿和 11 名配方奶喂养且属于对照组的婴儿。我们进行了一项比较宏基因组分析，以研究不同喂养方式和是否补充维生素 D 对婴儿肠道微生物群的分布和多样性的影响。我们的结果表明，婴儿肠道微生物群的特征不仅取决于喂养类型，还取决于营养素的摄入，并且表明维生素 D 在调节婴儿肠道微生物群方面起着重要作用，特别是增加了母乳喂养婴儿中益生菌的比例。
