Alsaegh Mohammed Amjed, Varma Sudhir Rama, Altaie Alaa Muayad, Zhu Shengrong
Department of Oral and Craniofacial Health Sciences, College of Dental Medicine, University of Sharjah, Sharjah, UAE.
Department of Oral and Maxillofacial Surgery, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P.R. China.
J Oral Microbiol. 2021 Jan 19;13(1):1874699. doi: 10.1080/20002297.2021.1874699.
The factor behind the activation of the remnant odontogenic tissues and development of odontogenic cysts and tumors is poorly understood.This study aimed to investigate the presence of human cytomegalovirus (HCMV) in dentigerous cyst (DC), odontogenic keratocyst (OKC), and ameloblastoma (AB). The study included 41 samples, which distributed into DC (n=13), OKC (n=12), and AB (n=16). Conventional PCR assay and IHC analysis were used to detect the HCMV-DNA and HCMV glycoprotein B (HCMV-gB) respectively. HCMV-DNA was detected in 10 samples (62.5%) of AB, four samples (30.8%) of DC, and three samples (25 %) of OKC respectively (χ test = 1.195, p= 0.247). Meanwhile, HCMV-gB was found in 12 (75%) of AB, in 2 (15.4%) of DC, and absent in OKC (0.0%) (χ test = 4.122, p= 0.042). The high prevalence of HCMV inside the odontogenic epithelium of AB could indicate a possible role of the virus in the oncogenesis and/or oncomodulation of the AB. Additionally, we recommend the IHC for the detection of HCMV in the odontogenic tumors like AB.
残余牙源性组织激活以及牙源性囊肿和肿瘤发生背后的因素目前尚不清楚。本研究旨在调查人巨细胞病毒（HCMV）在含牙囊肿（DC）、牙源性角化囊肿（OKC）和成釉细胞瘤（AB）中的存在情况。该研究包括41个样本，分为DC组（n = 13）、OKC组（n = 12）和AB组（n = 16）。分别采用常规PCR检测法和免疫组化分析来检测HCMV-DNA和HCMV糖蛋白B（HCMV-gB）。在AB组的10个样本（62.5%）、DC组的4个样本（30.8%）和OKC组的3个样本（25%）中分别检测到HCMV-DNA（χ检验= 1.195，p = 0.247）。同时，在AB组的12个样本（75%）、DC组的2个样本（15.4%）中发现了HCMV-gB，而在OKC组中未发现（0.0%）（χ检验= 4.122,p = 0.042）。AB的牙源性上皮内HCMV的高感染率可能表明该病毒在AB的肿瘤发生和/或肿瘤调节中发挥了作用。此外，我们建议采用免疫组化法检测AB等牙源性肿瘤中的HCMV。
