Hwang Ju-Ae, Kim Jung Eun, Kim Hyeong Su, Park Junseong, Lee Jeong-Ho
Inland Aquaculture Research Center, National Institute of Fisheries Science (NIFS), Changwon 51688, Korea.
Dev Reprod. 2020 Dec;24(4):277-286. doi: 10.12717/DR.2020.24.4.277. Epub 2020 Dec 31.
The disease-causing koi herpes virus (KHV), also known as cyprinid herpesvirus-3 (CyHV-3), causes mass mortality of koi and carp. Koi () is a host for KHV, one of 12 virus species in the family. We examined the effects of KHV disease koi (KK), and on koi×red common carp (KR) and red common carp×koi (RK) cross, using a virus challenge test. The infected fish had clinical signs that included gill necrosis and skin lesions. The RK and KR were highly more resistant (cumulative mortality: RK; 6% and KR; 8%) to KHV infection than KK fish (cumulative mortality: 28%). KHV DNA was confirmed in the tissues of all dead fish in groups by use of polymerase chain reaction (PCR), and the presence of the KHV protein in kidney was confirmed by immunohistochemistry. Histological analysis showed severe gill lesions and fusion of the lamellae in KK fish, but less severe damage in RK fish. In immunohistochemistry analysis, the KHV protein localized in the cytoplasm of infected kidney cells of KK, but the cross groups had lower levels of KHV antigen. Our data indicate that the cross groups had increased resistance to KHV disease.
致病的锦鲤疱疹病毒（KHV），也称为鲤疱疹病毒3型（CyHV-3），可导致锦鲤和鲤鱼大量死亡。锦鲤（）是KHV的宿主，KHV是该科12种病毒之一。我们通过病毒攻毒试验，研究了感染KHV疾病的锦鲤（KK），以及锦鲤×红鲤（KR）和红鲤×锦鲤（RK）杂交组合对KHV感染的影响。受感染的鱼出现了包括鳃坏死和皮肤损伤在内的临床症状。与KK鱼（累积死亡率：28%）相比，RK和KR对KHV感染具有更高的抵抗力（累积死亡率：RK为6%，KR为8%）。通过聚合酶链反应（PCR）在各实验组所有死亡鱼的组织中均确认了KHV DNA的存在，并通过免疫组织化学确认了肾脏中KHV蛋白的存在。组织学分析显示，KK鱼的鳃出现严重病变且鳃小片融合，但RK鱼的损伤较轻。在免疫组织化学分析中，KHV蛋白定位于KK感染肾脏细胞的细胞质中，但杂交组合的KHV抗原水平较低。我们的数据表明，杂交组合对KHV疾病的抵抗力增强。
