Research Institute for Humanity and Nature (RIHN), Kyoto 603-8047, Japan.
Res Vet Sci. 2012 Aug;93(1):508-14. doi: 10.1016/j.rvsc.2011.06.004. Epub 2011 Jul 1.
Cyprinid herpesvirus 3 (CyHV-3) disease is a significant threat for common and koi carp cultivators and for freshwater ecosystems. To determine the prevalence of CyHV-3 in Japanese rivers, a nationwide survey of all national class-A rivers was undertaken in the Summer of 2008. The virus was concentrated from river water samples using the cation-coated filter method. CyHV-3 DNA was detected in 90 rivers, representing 90% of 103 successfully analysed rivers. More than 100,000 copies of CyHV-3 DNA per litre of sample were detected in four rivers, higher than that reported during the Yura River outbreak in 2007. For CyHV-3-positive rivers, the log CyHV-3 density was negatively correlated with the water temperature on the sampling date and positively correlated with the suspended solids and dissolved oxygen, which are annually averaged for each river. Our results demonstrate that virus detection using molecular biology techniques is a powerful tool for monitoring the presence of CyHV-3 in natural environments.
锦鲤疱疹病毒 3 型（CyHV-3）疾病对普通鲤鱼和锦鲤养殖者以及淡水生态系统构成重大威胁。为了确定 CyHV-3 在日本河流中的流行情况，2008 年夏季对所有国家 A 级河流进行了全国范围的调查。使用带正电荷的过滤方法从河水样本中浓缩病毒。在 103 条成功分析的河流中，有 90 条河流检测到 CyHV-3 DNA，占 90%。在四条河流中，每升样本中检测到超过 100,000 份 CyHV-3 DNA，高于 2007 年在浦和河暴发期间的报告。对于 CyHV-3 阳性河流，采样日期的病毒密度与水温和悬浮固体和溶解氧呈负相关，而与河流每年的平均溶解氧和悬浮固体呈正相关。我们的结果表明，使用分子生物学技术检测病毒是监测自然环境中 CyHV-3 存在的有力工具。
