Choo Jocelyn M, Rogers Geraint B
SAHMRI Microbiome Research Laboratory, Flinders University College of Medicine and Public Health, Adelaide, SA 5000, Australia.
Microbiome and Host Health, South Australia Health and Medical Research Institute, North Terrace, Adelaide, SA 5000, Australia.
iScience. 2021 Jan 12;24(2):102049. doi: 10.1016/j.isci.2021.102049. eCollection 2021 Feb 19.
Determining whether associations between gut microbiota characteristics and host physiology represent causal relationships is a fundamental challenge for microbiome research. We report a detailed investigation of microbiome assembly in C57BL/6 germ-free mice across a period of 70 days and compare the effects of single and multiple rounds of gavage, using both native and antibiotic-disrupted murine donor material. Recipients of the native microbiota did not achieve compositional stability until day 28 and persistent differences to donor microbiota remained until day 70. Performing multiple rounds of gavage significantly increased the cumulative number of detected taxa (mean increase: 10.4%) and compositional similarity to donor, and significantly reduced within-group variance (p < 0.05). Multiple rounds of gavage with antibiotic-disrupted microbiota provided no substantial benefit in relation to compositional similarity to donor or within-group variance. The process of donor microbiota establishment in recipient animals is necessary before experimentation commences and is considerably influenced by donor microbiota characteristics.
确定肠道微生物群特征与宿主生理之间的关联是否代表因果关系是微生物组研究的一项基本挑战。我们报告了对C57BL/6无菌小鼠70天内微生物组组装的详细研究，并比较了单次和多次灌胃的效果，使用天然和抗生素破坏的小鼠供体材料。天然微生物群的受体直到第28天才实现组成稳定性，与供体微生物群的持续差异一直持续到第70天。进行多轮灌胃显著增加了检测到的分类群的累积数量（平均增加：10.4%）以及与供体的组成相似性，并显著降低了组内方差（p<0.05）。用抗生素破坏的微生物群进行多轮灌胃在与供体的组成相似性或组内方差方面没有提供实质性益处。在实验开始前，受体动物中供体微生物群的建立过程是必要的，并且受到供体微生物群特征的显著影响。
