Wang Lina, Zhu Chenglin
College of Food Science and Technology, Southwest Minzu University, Chengdu 610041, China.
J Agric Food Chem. 2021 Feb 17;69(6):1831-1841. doi: 10.1021/acs.jafc.0c06587. Epub 2021 Feb 4.
We tested the hypothesis that the consumption of different milk lipids is one of the factors affecting metabolic response to lipid in the early life of infants. Neonatal piglets, as animal models, were stratified by the feeding mode (formula-fed, bovine-, caprine-, and human milk-fed). Lipidomic profiles of plasma and liver samples were detected using liquid chromatography-mass spectrometry (LC-MS). The results indicate that 31, 54, and 28 differential lipid species could be used as potential biomarkers for bovine milk, caprine milk, and infant formula-fed samples, respectively, and the main lipid classes screened in plasma were SM, PC, and PE, including PC(14:1/P-20:0) as the isoform of PC(34:1), which regulates the lipid metabolism gene peroxisome proliferator-activated receptor α, PPAR-α. SM(d15:1/22:0) was the common potential biomarker screened from all of the groups. The amounts of biomarkers screened from the caprine milk-fed liver samples were the highest, which had a significant effect on the distribution of SM, PI, and PA. Infant formula, bovine-, and caprine milk-fed samples had an obvious effect on the metabolism of glycerophospholipid and glycerol ester, especially TG (16:0/18:0/18:2).
食用不同的乳脂是影响婴儿早期对脂质代谢反应的因素之一。以新生仔猪作为动物模型，根据喂养方式（配方奶喂养、牛乳喂养、羊乳喂养和人乳喂养）进行分层。使用液相色谱 - 质谱联用仪（LC - MS）检测血浆和肝脏样本的脂质组学图谱。结果表明，分别有31种、54种和28种差异脂质种类可作为牛乳、羊乳和配方奶喂养样本的潜在生物标志物，血浆中筛选出的主要脂质类别为鞘磷脂（SM）、磷脂酰胆碱（PC）和磷脂酰乙醇胺（PE），其中PC(14:1/P - 20:0)作为PC(34:1)的异构体，可调节脂质代谢基因过氧化物酶体增殖物激活受体α（PPAR - α）。SM(d15:1/22:0)是所有组中共同筛选出的潜在生物标志物。从羊乳喂养的肝脏样本中筛选出的生物标志物数量最多，对鞘磷脂、磷脂酰肌醇（PI）和磷脂酸（PA）的分布有显著影响。配方奶、牛乳和羊乳喂养样本对甘油磷脂和甘油酯的代谢有明显影响，尤其是甘油三酯（TG，16:0/18:0/18:2）。
