Gurjar Malkhan Singh, Aggarwal Rashmi, Jain Shekhar, Sharma Sapna, Singh Jagmohan, Gupta Sangeeta, Agarwal Shweta, Saharan Mahender Singh
Fungal Molecular Biology Laboratory, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India.
Department of Biotechnology, Mandsaur University, Mandsaur, Madhya Pradesh 45800, India.
J Fungi (Basel). 2021 Feb 2;7(2):103. doi: 10.3390/jof7020103.
Karnal bunt of wheat is an internationally quarantined disease affecting trade, quality, and production of wheat. During 2015-2016, a severe outbreak of Karnal bunt disease occurred in north-western plain zone of India. The present study was undertaken to decipher genetic variations in Indian isolates of collected from different locations. Seven multilocus sequence fragments were selected to differentiate and characterize these isolates. A phylogenetic tree constructed based on pooled sequences of actin-related protein 2 (ARP2), β-tubulin (TUB), eukaryotic translation initiation factor 3 subunit A (EIF3A), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), histone 2B (H2B), phosphoglycerate kinase (PGK), and serine/threonine-protein kinase (STPK) showed that isolate KB-11 (Kaithal, Haryana) was highly conserved as it was located in cluster 1 and has the maximum sequence similarity with the reference strain. Other isolates in cluster 1 included KB-16 and KB-17, both from Uttar Pradesh, and KB-19 from Haryana. Isolates KB-07 (Jind, Haryana) and KB-18 (Mujaffar Nagar, Uttar Pradesh) were the most diverse and grouped in a subgroup of cluster 2. Maximum numbers of single nucleotide polymorphisms (SNPs) (675) were in the PGK gene across the isolates. The minimum numbers of SNPs (67) were in KB-11 (Kaithal, Haryana), while the maximum number of SNPs (165) was identified in KB-18, followed by 164 SNPs in KB-14. KB-18 isolate was found to be the most diverse amongst all isolates. This first study on multilocus sequence typing (MLST) revealed that the population of was highly diverse.
小麦印度腥黑穗病是一种国际检疫性病害，影响小麦贸易、品质和产量。2015 - 2016年期间，印度西北平原地区爆发了严重的印度腥黑穗病疫情。本研究旨在解析从不同地点收集的印度菌株的遗传变异。选择了七个多基因座序列片段来区分和表征这些菌株。基于肌动蛋白相关蛋白2（ARP2）、β - 微管蛋白（TUB）、真核翻译起始因子3亚基A（EIF3A）、甘油醛 - 3 - 磷酸脱氢酶（GAPDH）、组蛋白2B（H2B）、磷酸甘油酸激酶（PGK）和丝氨酸/苏氨酸蛋白激酶（STPK）的合并序列构建的系统发育树表明，菌株KB - 11（哈里亚纳邦凯萨尔）高度保守，因为它位于簇1中，与参考菌株的序列相似性最高。簇1中的其他菌株包括均来自北方邦的KB - 16和KB - 17，以及来自哈里亚纳邦的KB - 19。菌株KB - 07（哈里亚纳邦金德）和KB - 18（北方邦穆扎法尔讷格尔）最为多样，归为簇2的一个亚组。在所有菌株中，PGK基因的单核苷酸多态性（SNP）数量最多（675个）。KB - 11（哈里亚纳邦凯萨尔）的SNP数量最少（67个），而KB - 18中的SNP数量最多（165个），其次是KB - 14中的164个SNP。发现KB - 18菌株在所有菌株中最为多样。这项关于多基因座序列分型（MLST）的首次研究表明，该病菌种群具有高度多样性。
