Department of Obstetrics and Gynaecology, Peking University First Hospital, Beijing, China.
Beijing Key Laboratory of Maternal Fetal Medicine of Gestational Diabetes Mellitus, Beijing, China.
J Matern Fetal Neonatal Med. 2022 Aug;35(15):2915-2922. doi: 10.1080/14767058.2020.1812571. Epub 2021 Feb 4.
The characteristics of microbial community changes in pregnant women are still unclear. To investigate the changes in gut microbiota during pregnancy and after delivery in healthy women, we enrolled 47 healthy pregnant women who received obstetric care in our hospital from October 2016 to April 2017 and obtained their fecal samples at different time periods: T1 (11-13 W), T2 (23-28 W), and T3 (33-38 W) during pregnancy, and PP6W (6 weeks postpartum) and PP6M (6 months postpartum). Based on 16S rRNA gene sequencing results, there was no significant difference ( .05) in the index of alpha-diversity between the pregnancy and postpartum periods. Principal coordinate analysis indicated that gut microbiota clustering during the postpartum period was significantly different from that during pregnancy. Phylum-level comparison of species identified using T1, T2, T3, PP6W, and PP6M samples showed higher abundance of Actinobacteria, Firmicutes, and Proteobacteria, while the abundance of Bacteroidetes decreased. At the genus level, 31 types of bacteria were found to be significantly different among these five groups. , and showed higher abundance during pregnancy, while , group and showed higher abundance after delivery. Therefore, the diversity and function of the gut microbiota in healthy pregnant women remained unchanged during pregnancy; however, the composition of the intestinal microbiota in the postpartum period changed significantly. Our results provide the basis for in-depth studies of the composition of perinatal gut microbial communities in women.
孕妇肠道微生物群落变化的特征尚不清楚。为了研究健康孕妇在怀孕期间和分娩后的肠道微生物群变化，我们招募了 47 名在我院接受产科护理的健康孕妇，并在不同时期获得了她们的粪便样本：T1（11-13 周）、T2（23-28 周）和 T3（33-38 周）在怀孕期间，以及 PP6W（产后 6 周）和 PP6M（产后 6 个月）。基于 16S rRNA 基因测序结果，怀孕期间和产后的 alpha 多样性指标没有显著差异（ .05）。主坐标分析表明，产后期间肠道微生物群聚类与怀孕期间显著不同。使用 T1、T2、T3、PP6W 和 PP6M 样本鉴定的物种的门水平比较表明，放线菌门、厚壁菌门和变形菌门的丰度较高，而拟杆菌门的丰度降低。在属水平上，发现这五组中有 31 种细菌存在显著差异。在怀孕期间， 和 丰度较高，而在分娩后， 和 组和 丰度较高。因此，健康孕妇在怀孕期间的肠道微生物群多样性和功能保持不变；然而，产后肠道微生物群落的组成发生了显著变化。我们的研究结果为深入研究女性围产期肠道微生物群落的组成提供了基础。
