Sokhey J, Gupta C K, Sharma B, Singh H
Polio Vaccine Testing Laboratory, Central Research Institute, Kasauli, India.
Vaccine. 1988 Feb;6(1):12-3. doi: 10.1016/0264-410x(88)90006-0.
The stability of five batches of oral polio vaccine stored at -20, 4-8, 22 and 36 degrees C for 7, 14 and 21 days was studied. The virus titrations were performed by the standard macro-method. There was little loss in virus titre when samples were kept at -20 and 4-8 degrees C for 21 days, whereas the samples exposed to 36 degrees C for 21 days showed almost complete loss in virus titre. The average loss in virus titre in a year (log TCID50) was 0.47 at -20 degrees C and 0.65 at 4-8 degrees C when various samples were stored at these temperatures. At 22 degrees C the average loss in virus titre after 21 days was about 1.50. The samples subjected to ten cycles of freezing and thawing did not show any loss in virus titre. Likewise there was not much loss in virus titre in three samples stored at 4-8 degrees C for a year. Oral polio vaccine stabilized with magnesium chloride is quite a stable vaccine and maintenance of a proper cold chain is recommended for the delivery of a potent vaccine in countries with high ambient temperature.
研究了五批口服脊髓灰质炎疫苗分别在-20℃、4-8℃、22℃和36℃下储存7天、14天和21天的稳定性。病毒滴定采用标准宏观方法进行。当样品在-20℃和4-8℃下保存21天时，病毒滴度几乎没有损失，而暴露于36℃下21天的样品病毒滴度几乎完全丧失。当各种样品在这些温度下储存时，一年中病毒滴度的平均损失（log TCID50）在-20℃时为0.47，在4-8℃时为0.65。在22℃下，21天后病毒滴度的平均损失约为1.50。经过十次冻融循环的样品病毒滴度没有任何损失。同样，在4-8℃下储存一年的三个样品的病毒滴度也没有太大损失。用氯化镁稳定的口服脊髓灰质炎疫苗是一种相当稳定的疫苗，建议在环境温度较高的国家保持适当的冷链以提供有效的疫苗。
