Department of Pharmacy, University of Oslo, PO Box 1068 Blindern, 0316 Oslo, Norway.
Department of Pharmacy, Faculty of Health & Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, Denmark.
Bioanalysis. 2021 Feb;13(4):277-289. doi: 10.4155/bio-2020-0285. Epub 2021 Feb 5.
This article reviews the scientific literature on electromembrane extraction (EME) of peptides and amino acids. In EME, target analytes are extracted from aqueous sample, through a supported liquid membrane (organic) and into a microliter volume of aqueous buffer (acceptor). Experimental conditions and performance for EME of peptides and amino acids are reviewed and discussed in detail, providing readers with an overview and basic understanding of the subject. In addition, this review discuss the potential for future applications, and scientific questions that need to be addressed for EME of peptides and amino acids to be generally accepted. EME is under commercialization, and therefore we expect it will be an active area of research in the near future.
本文综述了电渗析萃取（EME）在肽和氨基酸分析中的应用。在 EME 中，目标分析物从水相样品中，通过支撑液膜（有机相）进入微升级的水相缓冲液（接受相）中。本文详细综述和讨论了 EME 萃取肽和氨基酸的实验条件和性能，为读者提供了对该主题的概述和基本了解。此外，本文还讨论了未来应用的潜力以及需要解决的科学问题，以使得 EME 广泛应用于肽和氨基酸的分析。EME 正在商业化，因此我们预计它将成为未来不久的一个活跃研究领域。
