College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Key Laboratory of Plant Immunity, Nanjing Agricultural University, Nanjing 210095, China.
Phytopathology. 2021 Mar;111(3):531-540. doi: 10.1094/PHYTO-07-20-0279-R. Epub 2021 Feb 4.
Glucose-6-phosphate isomerase (GPI) is ubiquitous in most organisms, catalyzing the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. In this study, we investigated biological and genetic functions of in the phytopathogen . We found that hyphal growth, conidial germination, and septa formation were significantly inhibited in deletion mutant ∆FgGPI. was also positively associated with glucose metabolism, ATP biosynthesis, and carbon source utilization. In addition, pyruvate production, deoxynivalenol (DON) biosynthesis, and virulence were reduced in ∆FgGPI. A coimmunoprecipitation assay demonstrated that FgGPI interacts with Fgβ. More importantly, the coimmunoprecipitation assay showed that carbendazim-resistant substitutions in β tubulin could reduce the interaction intensity between FgGPI and Fgβ, thereby increasing FgGPI expression and accelerating DON biosynthesis in carbendazim-resistant strains. Taken together, our work revealed the indispensable role of in fungal developmental processes, DON biosynthesis, and pathogenicity in .
葡萄糖-6-磷酸异构酶（GPI）在大多数生物中普遍存在，催化葡萄糖-6-磷酸和果糖-6-磷酸的可逆异构化。在这项研究中，我们研究了 在植物病原菌 中的生物学和遗传功能。我们发现菌丝生长、分生孢子萌发和隔膜形成在 ∆FgGPI 缺失突变体中受到显著抑制。 还与葡萄糖代谢、ATP 生物合成和碳源利用正相关。此外，丙酮酸产量、脱氧雪腐镰刀菌烯醇（DON）生物合成和毒力在 ∆FgGPI 中降低。共免疫沉淀测定表明 FgGPI 与 Fgβ相互作用。更重要的是，共免疫沉淀测定表明，β-微管蛋白中的苯并咪唑抗性取代可以降低 FgGPI 和 Fgβ 之间的相互作用强度，从而增加 FgGPI 的表达并加速苯并咪唑抗性菌株中 DON 的生物合成。总之，我们的工作揭示了 在真菌发育过程、DON 生物合成和致病性中的不可或缺作用。
