Arica Tugce A, Guzelgulgen Meltem, Yildiz Ahu Arslan, Demir Mustafa M
Department of Material Science and Engineering, Izmir Institute of Technology, 35430 Izmir, Turkey.
Department of Bioengineering, Izmir Institute of Technology, 35430 Izmir, Turkey.
Mater Sci Eng C Mater Biol Appl. 2021 Jan;120:111720. doi: 10.1016/j.msec.2020.111720. Epub 2020 Nov 13.
The development of biocompatible and transparent three-dimensional materials is desirable for corneal tissue engineering. Inspired from the cornea structure, gelatin methacryloyl-poly(2-hydroxymethyl methacrylate) (GelMA-p(HEMA)) composite hydrogel was fabricated. GelMA fibers were produced via electrospinning and covered with a thin layer of p(HEMA) in the presence of N,N'-methylenebisacrylamide (MBA) as cross-linker by drop-casting. The structure of resulting GelMA-p(HEMA) composite was characterized by spectrophotometry, microscopy, and swelling studies. Biocompatibility and biological properties of the both p(HEMA) and GelMA-p(HEMA) composite have been investigated by 3D cell culture, red blood cell hemolysis, and protein adsorption studies (i.e., human serum albumin, human immunoglobulin and egg white lysozyme). The optical transmittance of the GelMA-p(HEMA) composite was found to be approximately 70% at 550 nm. The GelMA-p(HEMA) composite was biocompatible with tear fluid proteins and convenient for cell adhesion and growth. Thus, as prepared hydrogel composite may find extensive applications in future for the development of corneal tissue engineering as well as preparation of stroma of the corneal material.
对于角膜组织工程而言，开发生物相容性好且透明的三维材料是很有必要的。受角膜结构的启发，制备了甲基丙烯酰化明胶-聚（甲基丙烯酸-2-羟甲酯）（GelMA-p(HEMA)）复合水凝胶。通过静电纺丝制备GelMA纤维，并在作为交联剂的N,N'-亚甲基双丙烯酰胺（MBA）存在下，通过滴铸法在其表面覆盖一层薄薄的p(HEMA)。通过分光光度法、显微镜检查和溶胀研究对所得GelMA-p(HEMA)复合材料的结构进行了表征。通过三维细胞培养、红细胞溶血和蛋白质吸附研究（即人血清白蛋白、人免疫球蛋白和蛋清溶菌酶），对p(HEMA)和GelMA-p(HEMA)复合材料的生物相容性和生物学特性进行了研究。发现GelMA-p(HEMA)复合材料在550nm处的透光率约为70%。GelMA-p(HEMA)复合材料与泪液蛋白具有生物相容性，便于细胞黏附和生长。因此，所制备的水凝胶复合材料未来可能在角膜组织工程的发展以及角膜材料基质的制备中得到广泛应用。
