Atef Asmaa Kh, Mostafa Tahia B, El-Sherif Hazem M
Chemistry Department, Faculty of Women for Art, Science and Education, Ain Shams University, Héliopolis, Cairo, Egypt.
Polymers and Pigments Department, National Research Centre, Cairo, Egypt.
Sci Rep. 2025 Mar 25;15(1):10224. doi: 10.1038/s41598-025-93040-2.
The development of biocompatible and hemocompatible materials is crucial for various biomedical applications. In this study, gelatin (Gel) was modified using glycidyl methacrylate (GMA) to create a photo-curable macromer (Gel-GMA), facilitating subsequent crosslinking via UV radiation. Additionally, a composite was prepared by incorporating graphene oxide (GO) into the modified gelatin matrix (Gel-GMA/GO). Structural and morphological analyses revealed macroporous or interconnected structures in the hydrogels and composites, resulting in high swelling capacities (> 1050%). Hemolysis testing demonstrated minimal hemolytic activity for both Gel-GMA and Gel-GMA/GO hydrogels, confirming their excellent hemocompatibility (0.54 and 0.50% respectively). Prothrombin time (PT) tests indicated negligible differences compared to normal blood, suggesting low thrombogenicity. The incorporation of GO reduced the PT to 12.9s. Furthermore, in vitro degradation studies under simulated blood conditions revealed moderate degradation rates) for Gel-GMA and Gel-GMA/GO hydrogels (37 and 18%, respectively) after 30 days. Viability assays on MRC-5 cells exposed to composite extracts up to 500 µg/ml showed consistent cell viability (more than 91.7%), with a slight reduction at higher concentrations. These findings underscore the potential of the hydrogels for applications such as vascular catheters, highlighting their biocompatibility, hemocompatibility, and controlled degradability.
生物相容性和血液相容性材料的开发对于各种生物医学应用至关重要。在本研究中，使用甲基丙烯酸缩水甘油酯（GMA）对明胶（Gel）进行改性，以制备一种可光固化的大分子单体（Gel-GMA），便于随后通过紫外线辐射进行交联。此外，通过将氧化石墨烯（GO）掺入改性明胶基质（Gel-GMA/GO）中制备了一种复合材料。结构和形态分析表明，水凝胶和复合材料中存在大孔或相互连接的结构，导致高溶胀能力（>1050%）。溶血试验表明，Gel-GMA和Gel-GMA/GO水凝胶的溶血活性均极低，证实了它们具有优异的血液相容性（分别为０.54%和０.50%）。凝血酶原时间（PT）测试表明，与正常血液相比差异可忽略不计，表明血栓形成性较低。GO的掺入使PT降至12.9秒。此外，在模拟血液条件下的体外降解研究表明，Gel-GMA和Gel-GMA/GO水凝胶在30天后具有中等降解率（分别为37%和18%）。对暴露于浓度高达500µg/ml的复合提取物中的MRC-5细胞进行的活力测定显示，细胞活力一致（超过91.7%），在较高浓度下略有下降。这些发现强调了水凝胶在血管导管等应用中的潜力，突出了它们的生物相容性、血液相容性和可控降解性。
