Reproductive Medical Centre, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Department of Urology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Andrologia. 2021 May;53(4):e14004. doi: 10.1111/and.14004. Epub 2021 Feb 7.
To evaluate the effect of resveratrol in rats with chronic prostatitis, 24 rats were randomly divided into the negative control, vehicle-treated and resveratrol groups. The rats in the vehicle-treated group and the resveratrol group were injected subcutaneously with 17-β-oestradiol (0.25 mg/kg) daily for 6 weeks while the rats in the control group were injected with equivalent normal saline. From the 45th day, the rats in the resveratrol group were given resveratrol (10 mg/kg) by gavage per day while the rest rats were given normal saline. After 55 days, all the rats were sacrificed and the prostatic tissue was removed. Morphological changes were examined by light microscope after H&E staining. The expressions of IL-6, IL-8 and TNF-α were determined through ELISA and immunohistochemical staining. As a result, significant inflammatory cell infiltration and fibroblastic hyperplasia were observed in prostatic stroma in the vehicle-treated group compared with the negative control group, as well as the high expression of IL-6, IL-8 and TNF-α. After resveratrol treatment, inflammatory cell infiltration and fibroblastic hyperplasia were shown prominently reduced. Meanwhile, the expression of IL-6, IL-8 and TNF-α was significantly suppressed. For conclusion, resveratrol could attenuate the prostatic inflammation and downregulate the expression of IL-6, IL-8 and TNF-α in rat with oestradiol-induced chronic prostatitis.
为了评估白藜芦醇对慢性前列腺炎大鼠的影响，将 24 只大鼠随机分为阴性对照组、溶媒处理组和白藜芦醇组。溶媒处理组和白藜芦醇组大鼠每日皮下注射 17-β-雌二醇（0.25mg/kg），共 6 周；对照组大鼠注射等量的生理盐水。从第 45 天开始，白藜芦醇组大鼠每天通过灌胃给予白藜芦醇（10mg/kg），其余大鼠给予生理盐水。55 天后，所有大鼠被处死并取出前列腺组织。通过 H&E 染色后，在光镜下检查形态学变化。通过 ELISA 和免疫组化染色测定 IL-6、IL-8 和 TNF-α 的表达。结果显示，与阴性对照组相比，溶媒处理组大鼠前列腺基质中可见明显的炎症细胞浸润和纤维母细胞增生，以及 IL-6、IL-8 和 TNF-α 的高表达。经过白藜芦醇治疗后，炎症细胞浸润和纤维母细胞增生明显减少，同时 IL-6、IL-8 和 TNF-α 的表达也显著受到抑制。综上所述，白藜芦醇可以减轻雌二醇诱导的慢性前列腺炎大鼠的前列腺炎症，并下调 IL-6、IL-8 和 TNF-α 的表达。
