罗汉果果实和染料木黄酮通过防止脂质积累、胰岛素抵抗及增强β细胞功能来改善大鼠2型糖尿病。
Mormodica charantia L. fruit and Genistein ameliorates type 2 diabetes in rats by preventing lipid accumulation, insulin resistance and enhancing beta cell function.
作者信息
Makena Wusa, Hambolu Joseph O, Timbuak James A, Umana Uduak E, Iliya Abdullahi I, Dibal Nathan I
机构信息
Department of Human Anatomy, Ahmadu Bello University, Zaria, Kaduna State Nigeria.
Department of Human Anatomy, University of Maiduguri, Maiduguri, Borno State Nigeria.
出版信息
J Diabetes Metab Disord. 2020 Oct 3;19(2):1303-1310. doi: 10.1007/s40200-020-00648-4. eCollection 2020 Dec.
PURPOSE
The present study was aimed at evaluating the role of fruit and Genistein on beta cell, insulin resistance/sensitivity and lipid profile in type 2 diabetic rats.
METHODS
Thirty-five (35) albino rats were divided into seven (7) groups of 5 rats each comprising of five (5) non-diabetic and thirty (30) diabetic rats. Groups 1 and 2 served as the normal control and diabetic control groups respectively and received distill water, groups 3 and 4 received at 250 mg/kg and 500 mg/kg respectively Groups 5 and 6 received Genistein at 10 mg/kg and 20 mg/kg respectively while group 7 received Metformin at 500 mg/kg the experiment lasted for four weeks. All the rats were euthanized at the end of the fourth week.
RESULTS
Lipid profile, glucose and insulin levels were determined from the analysis of serum parameters and the histology of the pancreas. A significant reduction ( < 0.05) in blood glucose levels was noticed in rats that received (MC) and genistein when compared with diabetic control rats. A significant decrease ( < 0.05) in cholesterol, triglyceride, low density lipoprotein (LDL) and very low density lipoprotein (VLDL) levels were also noted in rats that received MC and Genistein when compared with the diabetic control rats. MC and Genistein significantly increased ( < 0.05) serum insulin level compared to the diabetic control rats. MC and Genistein significantly decreased ( < 0.05) homeostatic model assessment-insulin resistance (HOMA-IR) level compared with the diabetic control group. Pancreas of rats that received MC and Genistein showed regenerating beta-cells.
CONCLUSION
fruit and Genistein were able to enhance beta cell function and prevent lipid accumulation and insulin resistance in type 2 diabetic rats.
目的
本研究旨在评估水果和金雀异黄素对2型糖尿病大鼠β细胞、胰岛素抵抗/敏感性及血脂谱的作用。
方法
35只白化大鼠分为7组，每组5只，其中包括5只非糖尿病大鼠和30只糖尿病大鼠。第1组和第2组分别作为正常对照组和糖尿病对照组，给予蒸馏水；第3组和第4组分别给予水果提取物250mg/kg和500mg/kg；第5组和第6组分别给予金雀异黄素10mg/kg和20mg/kg；第7组给予二甲双胍500mg/kg。实验持续4周。在第4周结束时对所有大鼠实施安乐死。
结果
通过血清参数分析和胰腺组织学检查测定血脂谱、血糖和胰岛素水平。与糖尿病对照组大鼠相比，接受水果提取物（MC）和金雀异黄素的大鼠血糖水平显著降低（<0.05）。与糖尿病对照组大鼠相比，接受MC和金雀异黄素的大鼠胆固醇、甘油三酯、低密度脂蛋白（LDL）和极低密度脂蛋白（VLDL）水平也显著降低（<0.05）。与糖尿病对照组大鼠相比，MC和金雀异黄素显著提高（<0.05）血清胰岛素水平。与糖尿病对照组相比，MC和金雀异黄素显著降低（<0.05）稳态模型评估胰岛素抵抗（HOMA-IR）水平。接受MC和金雀异黄素的大鼠胰腺显示β细胞再生。
结论
水果和金雀异黄素能够增强2型糖尿病大鼠的β细胞功能，预防脂质蓄积和胰岛素抵抗。
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