Ha Jinhee, Bharti Dinesh, Kang Young-Hoon, Lee Sang-Yeob, Oh Seong-Ju, Kim Saet-Byul, Jo Chan-Hee, Son Jang-Ho, Sung Iel-Yong, Cho Yeong-Cheol, Rho Gyu-Jin, Park Jeong-Kil
Department of Dentistry, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan, Republic of Korea.
Department of Theriogenology and Biotechnology, College of Veterinary Medicine and Research Institute of Life Science, Gyeongsang National University, Jinju, Republic of Korea.
Biomed Res Int. 2021 Jan 22;2021:8858412. doi: 10.1155/2021/8858412. eCollection 2021.
Previous studies have shown that mesenchymal stem cells (MSCs) derived from various tissue sources can be differentiated into smooth muscle-like cells (SMLCs) in vitro. In this paper, dental pulp-derived mesenchymal stem cells (DPSCs) were evaluated for their differentiation ability towards smooth muscle-like cells (SMLCs) under the effect of widely used cytokines (TGF-1 and PDGF-BB) with special focus on different culturing environments. For this purpose, both the commercially used culturing plates (Norm-c) and 0.1% gelatin-precoated (Gel-c) plates were used. Isolated cells displayed plastic adherence, pluripotency and cell surface marker profiling, and adipogenic and osteogenic differentiation potential with lineage specific marker expression. Differentiated cells induced under different culturing plates showed successful differentiation into SMLCs by positively expressing smooth muscle cell (SMC) specific markers both at the mRNA and protein levels. Gelatin coating could substantially enhance DPSC differentiation potential than Norm-c-induced cells. However, the absence of mature marker MHY-11 by immunostaining results from all treatment groups further indicated the development of immature and synthetic SMLCs. Finally, it was concluded that DPSC differentiation ability into SMLCs can be enhanced under cytokine treatment as well as by altering the cellular niche by precoating the culturing plates with suitable substrates. However, to get fully functional, contractile, and mature SMLCs, still many different cytokine cocktail combinations and more suitable coating substrates will be needed.
先前的研究表明，源自各种组织来源的间充质干细胞（MSCs）在体外可分化为平滑肌样细胞（SMLCs）。在本文中，评估了牙髓来源的间充质干细胞（DPSCs）在广泛使用的细胞因子（TGF-1和PDGF-BB）作用下向平滑肌样细胞（SMLCs）的分化能力，并特别关注不同的培养环境。为此，使用了市售的培养板（Norm-c）和0.1%明胶预包被（Gel-c）的培养板。分离的细胞表现出贴壁生长、多能性和细胞表面标志物谱，以及具有谱系特异性标志物表达的成脂和成骨分化潜能。在不同培养板上诱导分化的细胞通过在mRNA和蛋白质水平上阳性表达平滑肌细胞（SMC）特异性标志物，成功分化为SMLCs。与Norm-c诱导的细胞相比，明胶包被可显著增强DPSC的分化潜能。然而，所有处理组免疫染色结果均未出现成熟标志物MHY-11，这进一步表明了未成熟和合成型SMLCs的形成。最后得出结论，在细胞因子处理以及通过用合适的底物预包被培养板来改变细胞微环境的情况下，DPSC向SMLCs的分化能力可以增强。然而，要获得功能完全正常、可收缩且成熟的SMLCs，仍需要许多不同的细胞因子鸡尾酒组合和更合适的包被底物。
