Liu Yaqun, Lin Yea-Lih, Pasero Philippe, Chen Chun-Long
Institut Curie, PSL Research University, CNRS, UMR3244, Sorbonne Université, Paris, France.
Institut de Génétique Humaine, CNRS et Université de Montpellier, Montpellier, France.
Mol Cell Oncol. 2020 Dec 7;8(1):1843951. doi: 10.1080/23723556.2020.1843951.
R-loops have both positive and negative impacts on chromosome functions. To identify toxic R-loops, we mapped RNA:DNA hybrids, markers of replication fork stalling and DNA double-strand breaks along the human genome. This analysis indicates that transient replication fork pausing occurs at the transcription termination sites of highly expressed genes enriched in R-loops and prevents head-on conflicts with transcription, in a topoisomerase I-dependent manner.
R环对染色体功能既有积极影响，也有消极影响。为了识别有害的R环，我们绘制了RNA:DNA杂交体、复制叉停滞标记以及人类基因组中的DNA双链断裂图谱。该分析表明，短暂的复制叉暂停发生在富含R环的高表达基因的转录终止位点，并以拓扑异构酶I依赖的方式防止与转录发生迎头冲突。
