Thangwana Andries, Gwata Eastonce T, Zhou Marvelous M
University of Venda, School of Agriculture, Department of Plant Production, P. Bag X5050, Thohoyandou, 0950, South Africa.
South African Sugarcane Research Institute, P. Bag X02, Mount Edgecombe, 4300, South Africa.
Heliyon. 2021 Jan 29;7(1):e06103. doi: 10.1016/j.heliyon.2021.e06103. eCollection 2021 Jan.
Tepary bean is an important food legume. The genetic improvement of the crop is limited by a narrow genetic base but this genetic base can be broadened through induced mutagenesis. This study was designed to determine the (i) effects of chemical mutagenesis using ethyl methane sulfonate (EMS) on M seedlings (ii) the adult plant performance of M - M generations and (iii) the relationships between growth attributes at the seedling and adult plant stage. Mutagenized seed with varying doses of EMS was germinated at room temperature in order to raise M seedlings. There were highly significant (P < 0.01) differences due to dose effects among the seedlings. The highest LD (3.37 % EMS v/v) was observed for 'Genotype 3'. Under field conditions, all the three factors influenced the plant performance. The results demonstrated the potential of EMS to induce genotypic variation and desirable agronomic traits in tepary bean.
tepary豆是一种重要的食用豆类。该作物的遗传改良受到狭窄遗传基础的限制，但可以通过诱变来拓宽这一遗传基础。本研究旨在确定：（i）使用甲基磺酸乙酯（EMS）进行化学诱变对M1代幼苗的影响；（ii）M1 - M2代成年植株的表现；（iii）幼苗期和成年植株期生长特性之间的关系。将不同剂量EMS诱变处理的种子在室温下萌发，以培育M1代幼苗。由于剂量效应，幼苗之间存在极显著（P < 0.01）差异。“基因型3”的最高致死剂量（3.37% EMS v/v）被观察到。在田间条件下，所有这三个因素都影响植株表现。结果证明了EMS在tepary豆中诱导基因型变异和理想农艺性状的潜力。
