Rodriguez Jae Joseph Russell B, Laude Rita P, De Ungria Maria Corazon A
DNA Analysis Laboratory, Natural Sciences Research Institute, University of the Philippines Diliman, Quezon City, 1101, Philippines.
Genetics and Molecular Biology Division, Institute of Biological Sciences, College of Arts and Sciences, University of the Philippines Los Baños, Laguna, 4031, Philippines.
Forensic Sci Int Synerg. 2021 Jan 11;3:100133. doi: 10.1016/j.fsisyn.2021.100133. eCollection 2021.
In the Philippines, more than 7000 cases of sexual assault are reported annually. DNA technology is a powerful tool in identifying assailants. However, it is not routinely used in sexual assault investigations due to insufficient government support to cover the high cost of DNA testing and the absence of a national system for sample collection, handling, storage, and DNA testing of biological evidence. In itself, the nature of sexual assault samples containing DNA mixtures presents challenges to laboratory methods and interpretation of results. The sample recovered from the victim may only contain trace amounts of the assailant's DNA, may have degraded due to prolonged storage in ambient conditions which is warm and humid in the tropics, or contaminated with inhibitors, such as in anal swabs. Hence, a closer evaluation of the processes of evidence collection and DNA testing is needed to increase the likelihood of success in generating conclusive results. In this paper, we propose an integrated system for DNA testing of biological samples collected from sexual assault victims considering the limitations of resources and the prevailing warm climate. Recommendations in this work should provide basis for formulating national guidelines for DNA analysis in aid of criminal investigations. The proposed scheme can be adopted by forensic DNA laboratories in the Philippines and in other countries facing similar challenges.
在菲律宾,每年报告的性侵犯案件超过7000起。DNA技术是识别袭击者的有力工具。然而,由于政府支持不足,无法支付DNA检测的高昂费用,且缺乏生物证据样本采集、处理、存储和DNA检测的国家系统,DNA技术并未在性侵犯调查中常规使用。就其本身而言,含有DNA混合物的性侵犯样本的性质给实验室方法和结果解读带来了挑战。从受害者身上采集的样本可能只含有微量的袭击者DNA,可能因在热带地区温暖潮湿的环境中长时间储存而降解,或者被抑制剂污染,比如肛门拭子。因此,需要对证据采集和DNA检测过程进行更仔细的评估,以提高得出确凿结果的成功率。在本文中,考虑到资源限制和普遍的温暖气候,我们提出了一个针对从性侵犯受害者身上采集的生物样本进行DNA检测的综合系统。本研究中的建议应为制定刑事调查辅助DNA分析的国家指南提供依据。菲律宾以及面临类似挑战的其他国家的法医DNA实验室可以采用该提议的方案。