Laboratorio de Toxicología de Productos Naturales, Departamento de Farmacia, IPN, Escuela Nacional de Ciencias Biológicas, Av. Wilfrido Massieu, Gustavo A. Madero, 07738 Ciudad de México, México; Laboratorio de Investigación Biomédica en Productos Naturales, Carrera de Medicina, UNAM, FES-Iztacala, Avenida de los Barrios Número 1, Colonia Los Reyes Iztacala, 54090 Tlalnepantla, Estado de México, México.
Laboratorio de Toxicología de Productos Naturales, Departamento de Farmacia, IPN, Escuela Nacional de Ciencias Biológicas, Av. Wilfrido Massieu, Gustavo A. Madero, 07738 Ciudad de México, México.
Biomed Pharmacother. 2021 May;137:111345. doi: 10.1016/j.biopha.2021.111345. Epub 2021 Feb 5.
The aim of this work was to evaluate the gastroprotective activity of a Mexican propolis on indomethacin-induced gastric ulcers in a mouse model. The following contents of the ethanolic extract of propolis of Chihuahua (EEPCh) were determined: antioxidant activity (SA), total phenolic content (TPC), total flavonoid content (TFC), and chemical composition by HPLC-DAD and HPLC-MS, as well as acute toxicity by OECD Guideline 423. Gastric lesions were induced by intragastric indomethacin treatment in male ICR mice. As the positive control, omeprazole was administered, and three doses of EEPCh were evaluated (50, 150 and 300 mg/kg). Gastric mucosal injury, histological changes and mucosal content were evaluated by means of H&E and PAS staining. For homogenized gastric tissues, the following were evaluated: TBARS, MPO, and PGE levels; SOD and GPx antioxidant enzymatic activity; and the concentrations of the proinflammatory cytokines, TNF-α, IL-1β and IL-6. EEPCh had a significant SA of 41.55 µg/mL. The TPC of EEPCh was 860 mg GAE/g, and its TFC was 49.58 mg QE/g. Different phenolic compounds were identified in the extract and were not toxic. The EEPCh doses decreased mucosal damage and histological injuries, maintained the mucosal content and reduced the TBARS, MPO and concentrations of proinflammatory cytokines in gastric ulcer tissues. The 150 and 300 mg/kg doses increased the SOD activity and maintained the PGE content. Only the 300 mg/kg dose increased the GPx activity. The results of this study suggest that EEPCh displays gastroprotective effects by means of its antioxidant activity and anti-inflammatory effects and promotes ulcer protection through the maintenance of mucosal content and PGE levels.
本工作旨在评估墨西哥蜂胶在吲哚美辛诱导的小鼠胃溃疡模型中的胃保护活性。对来自奇瓦瓦州的蜂胶乙醇提取物（EEPCh）的以下内容进行了测定：抗氧化活性（SA）、总酚含量（TPC）、总黄酮含量（TFC）、HPLC-DAD 和 HPLC-MS 的化学成分分析，以及 OECD 指南 423 的急性毒性。通过胃内给予吲哚美辛诱导雄性 ICR 小鼠胃损伤。作为阳性对照，给予奥美拉唑，并评估了 EEPCh 的三个剂量（50、150 和 300mg/kg）。通过 H&E 和 PAS 染色评估胃黏膜损伤、组织学变化和黏膜含量。对于匀浆胃组织，评估了以下指标：TBARS、MPO 和 PGE 水平；SOD 和 GPx 抗氧化酶活性；以及促炎细胞因子 TNF-α、IL-1β 和 IL-6 的浓度。EEPCh 具有显著的 41.55µg/mL 的 SA。EEPCh 的 TPC 为 860mg GAE/g，TFC 为 49.58mg QE/g。在提取物中鉴定出不同的酚类化合物，且无毒性。EEPCh 剂量可降低黏膜损伤和组织学损伤，维持黏膜含量，并降低胃溃疡组织中的 TBARS、MPO 和促炎细胞因子浓度。150 和 300mg/kg 剂量可增加 SOD 活性并维持 PGE 含量。只有 300mg/kg 剂量可增加 GPx 活性。本研究结果表明，EEPCh 通过抗氧化和抗炎作用发挥胃保护作用，并通过维持黏膜含量和 PGE 水平促进溃疡保护。
