Mahami-Oskouei M, Ghabouli-Mehrabani N, Miahipour A, Fallah E, Shahbazi A, Mazhari N, Hamidi F
Tabriz Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Parasitology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Trop Biomed. 2015 Dec 1;32(4):717-725.
Hydatidosis is one of the most important zoonotic parasitic diseases caused by the larval stage of Echinococcus granulosus which causes great health and economic losses. The aim of this study was to use the sequencing method to evaluate genotypes of E. granulosus isolated from humans and bovines using mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. The samples were taken in the East Azerbaijan Province, Northwest Iran. Overall, 26 hydatid cyst samples (10 human and 16 cattle isolates) were collected. DNA extraction was taken from the protoscoleces of human and germinal layer of bovine samples. PCR was performed using the mitochondrial cytochrome c oxidase subunit 1(cox1) gene, and then it was sequenced. Sequences were analyzed for identification of their genotypes. All 16 bovine isolates were recognized as G1 genotypes (sheep strain) and G1B subtypes. Out of ten human host samples, seven isolates were G1B subtypes, and three samples were identified as G3 genotypes. The results of this study showed that G1 and especially G1B are the predominant genotype and subtype in humans and cattle in Northwest Iran.
包虫病是由细粒棘球绦虫幼虫阶段引起的最重要的人畜共患寄生虫病之一，会造成巨大的健康和经济损失。本研究的目的是使用测序方法，利用线粒体细胞色素c氧化酶亚基1（cox1）基因评估从人和牛分离出的细粒棘球绦虫的基因型。样本采自伊朗西北部的东阿塞拜疆省。总共收集了26个包虫囊肿样本（10个人类样本和16个牛样本）。从人类原头蚴和牛样本的生发层提取DNA。使用线粒体细胞色素c氧化酶亚基1（cox1）基因进行PCR，然后进行测序。对序列进行分析以鉴定其基因型。所有16个牛样本均被鉴定为G1基因型（绵羊株）和G1B亚型。在10个人类宿主样本中，7个样本为G1B亚型，3个样本被鉴定为G3基因型。本研究结果表明，G1尤其是G1B是伊朗西北部人和牛中的主要基因型和亚型。
