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伊朗法尔斯省基于线粒体基因测序的人临床手术肺包虫病基因型分离株。

Pulmonary hydatidosis genotypes isolates from human clinical surgery based on sequencing of mitochondrial genes in Fars, Iran.

机构信息

Department of Surgery, Shiraz University of Medical Sciences, Shiraz, Iran.

Thoracic and Vascular Surgery Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

出版信息

J Cardiothorac Surg. 2021 Jun 7;16(1):167. doi: 10.1186/s13019-021-01547-2.

DOI:10.1186/s13019-021-01547-2
PMID:34099002
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8186107/
Abstract

BACKGROUND

Cystic echinococcosis (CE)/hydatidosis is an important neglected parasitic zoonotic disease caused by the metacestode of Echinococcus granulosus s.l. The present study was designed to identify the pulmonary CE species/genotypes in isolated human underwent to surgery in our center in Southern Iran.

METHODS

The study population of this study were all patients in Fars province who were admitted to Namazi Hospitals for pulmonary hydatid cyst surgery. Thoracic surgery was performed in the thoracic ward and the cyst/s was removed by open surgery via posterolateral or lateral thoracotomy. DNA was extracted from the germinal layer or the protoscoleces. PCR technique was performed using the cytochrome C oxidase subunit1 (cox1) gene, and the products were sequenced.

RESULTS

A total of 32 pulmonary hydatid cyst samples were collected from 9 (28%) female and 23 (72%) male aged from 4 to 74 years old. A total of 18(56%) cyst/s were in the left lobe and 14 (44%) cysts in the right lobe. Sequence analysis of the cysts showed that 24 samples (75%) were E. granulosus s.s (G1-G3) genotype and 8 (25%) were E. canadensis (G6/G7) genotype.

CONCLUSION

E.granulosus s.s genotype was the most prevalent genotype followed by E. canadensis (G6/G7) genotype. There was no significant statistical correlation between cysts' size, location, genotype strain, and patients' age and gender.

摘要

背景

包虫病（CE）/包虫病是一种由细粒棘球绦虫的囊尾蚴引起的重要被忽视的寄生虫性人畜共患病。本研究旨在鉴定在伊朗南部我们中心接受手术的孤立性人类肺 CE 种/基因型。

方法

本研究的研究人群为法尔斯省所有因肺包虫囊肿手术而入院的患者。胸腔手术在胸腔病房进行，通过后外侧或外侧开胸切开术切除囊肿/囊。从生殖层或原头节中提取 DNA。使用细胞色素 C 氧化酶亚基 1 (cox1) 基因进行 PCR 技术，对产物进行测序。

结果

共采集了 32 个来自 9 名（28%）女性和 23 名（72%）男性的肺包虫囊肿样本，年龄从 4 岁到 74 岁不等。共有 18 个（56%）囊肿/囊在左叶，14 个（44%）在右叶。对囊肿的序列分析表明，24 个样本（75%）为细粒棘球绦虫 s.s 基因型（G1-G3），8 个（25%）为加拿大棘球绦虫（G6/G7）基因型。

结论

细粒棘球绦虫 s.s 基因型是最常见的基因型，其次是加拿大棘球绦虫（G6/G7）基因型。囊肿大小、位置、基因型菌株以及患者年龄和性别之间无显著统计学相关性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cab2/8186107/aa068a0d24d8/13019_2021_1547_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cab2/8186107/cf842a7e0c65/13019_2021_1547_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cab2/8186107/784c87983d52/13019_2021_1547_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cab2/8186107/3b4069bda1c1/13019_2021_1547_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cab2/8186107/aa068a0d24d8/13019_2021_1547_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cab2/8186107/cf842a7e0c65/13019_2021_1547_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cab2/8186107/784c87983d52/13019_2021_1547_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cab2/8186107/3b4069bda1c1/13019_2021_1547_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cab2/8186107/aa068a0d24d8/13019_2021_1547_Fig4_HTML.jpg

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