Walker R A, Camplejohn R S
Department of Pathology, Leicester Royal Infirmary, UK.
Br J Cancer. 1988 Mar;57(3):281-3. doi: 10.1038/bjc.1988.60.
The reactivity of 95 breast carcinomas with the antibody Ki-67, which recognises a nuclear antigen in proliferating cells, has been assessed and compared to their histological grade and, for 47 tumours, DNA index and S-phase content. The effects of freezing and section handling on the stability of the nuclear antigen have been assessed. Evidence of nuclear staining was seen in 56% of carcinomas, with a range of positive cells from less than 1% to 60%. Cytoplasmic rather than nuclear staining was observed in 26% and 18% of carcinomas were negative. A significant correlation was observed between the presence of nuclear staining and poorer histological grade and higher S-phase content, and between the percentage of positive nuclei and S-phase content, but not grade. Three groups of carcinomas were identified: those in which Ki-67 reactivity, grade and S-phase content were similar; ones in which there was prominent nuclear reactivity with Ki-67 but low grade and S-phase content; and a group showing the converse. These patients will be followed to assess which of these three markers of proliferation is of greatest prognostic value.
对95例乳腺癌与Ki-67抗体的反应性进行了评估，该抗体可识别增殖细胞中的一种核抗原，并将其与组织学分级进行比较，对47例肿瘤还比较了DNA指数和S期含量。评估了冷冻和切片处理对核抗原稳定性的影响。56%的癌可见核染色证据，阳性细胞范围从不到1%至60%。26%的癌观察到细胞质而非核染色，18%的癌为阴性。核染色的存在与较差的组织学分级和较高的S期含量之间，以及阳性核百分比与S期含量之间存在显著相关性，但与分级无关。确定了三组癌：Ki-67反应性、分级和S期含量相似的癌；Ki-67核反应性突出但分级和S期含量低的癌；以及呈现相反情况的一组癌。将对这些患者进行随访，以评估这三种增殖标志物中哪一种具有最大的预后价值。
