Adzitey Frederick, Huda Nurul, Shariff Amir Husni Mohd
Department of Food Science and Technology, University for Development Studies, P. O. Box TL 1882 Tamale, Ghana.
Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Malaysia.
Microorganisms. 2021 Feb 5;9(2):326. doi: 10.3390/microorganisms9020326.
Meat is an important food source that can provide a significant amount of protein for human development. The occurrence of bacteria that are resistant to antimicrobials in meat poses a public health risk. This study evaluated the occurrence and antimicrobial resistance of () isolated from raw meats, ready-to-eat (RTE) meats and their related samples in Ghana. was isolated using the USA-FDA Bacteriological Analytical Manual and phenotypic antimicrobial susceptibility test was performed by the disk diffusion method. Of the 200 examined meats and their related samples, 38% were positive for . Notably, was highest in raw beef (80%) and lowest in RTE pork (0%). The 45 isolates were resistant ≥ 50% to amoxicillin, trimethoprim and tetracycline. They were susceptible to azithromycin (87.1%), chloramphenicol (81.3%), imipenem (74.8%), gentamicin (72.0%) and ciprofloxacin (69.5%). A relatively high intermediate resistance of 33.0% was observed for ceftriaxone. from raw meats, RTE meats, hands of meat sellers and working tools showed some differences and similarities in their phenotypic antimicrobial resistance patterns. Half (51.1%) of the isolates exhibited multidrug resistance. The isolates showed twenty-two different resistant patterns, with a multiple antibiotic resistance index of 0.0 to 0.7. The resistant pattern amoxicillin (A, = 6 isolates) and amoxicillin-trimethoprim (A-TM, = 6 isolates) were the most common. This study documents that raw meats, RTE meats and their related samples in Ghana are potential sources of antimicrobial-resistant and pose a risk for the transfer of resistant bacteria to the food chain, environment and humans.
肉类是一种重要的食物来源，可为人类发育提供大量蛋白质。肉类中出现对抗菌药物具有抗性的细菌会构成公共卫生风险。本研究评估了从加纳的生肉、即食（RTE）肉类及其相关样本中分离出的（）的出现情况和抗菌药物抗性。使用美国食品药品监督管理局（USA - FDA）细菌学分析手册分离（），并通过纸片扩散法进行表型抗菌药物敏感性试验。在所检测的200份肉类及其相关样本中，38%的样本（）呈阳性。值得注意的是，生牛肉中的（）含量最高（80%），即食猪肉中的含量最低（0%）。这45株（）分离株对阿莫西林、甲氧苄啶和四环素的耐药率≥50%。它们对阿奇霉素（87.1%）、氯霉素（81.3%）、亚胺培南（74.8%）、庆大霉素（72.0%）和环丙沙星（69.5%）敏感。头孢曲松的中间耐药率相对较高，为33.0%。来自生肉、即食肉类、肉类销售者的手部和工作工具的（）在其表型抗菌药物抗性模式上表现出一些差异和相似性。这些（）分离株中有一半（51.1%）表现出多重耐药性。这些（）分离株呈现出22种不同的耐药模式，多重抗生素耐药指数为0.0至0.7。耐药模式阿莫西林（A，6株）和阿莫西林 - 甲氧苄啶（A - TM，6株）最为常见。本研究记录表明，加纳的生肉、即食肉类及其相关样本是耐抗菌药物（）的潜在来源，并存在耐药细菌向食物链、环境和人类转移的风险。
