Njeru Sospeter N, Muema Jackson M
Department of Biochemistry, School of Health Sciences, Kisii University, P.O. Box 408-40200, Kisii, Kenya.
Division of Immunology, Paul-Ehrlich-Institute, Federal Institute for Vaccines and Biomedicines, Paul-Ehrlich-Straße 51-59, 63225, Langen, Hessen, Germany.
BMC Res Notes. 2021 Feb 9;14(1):57. doi: 10.1186/s13104-021-05472-4.
We and others have shown that Aspilia pluriseta is associated with various biological activities. However, there is a lack of information on its cytotoxicity. This has created an information gap about the safety of A. pluriseta extracts. As an extension to our recent publication on the antimicrobial activity and the phytochemical characterization of A. pluriseta root extracts, here we report on cytotoxicity of tested solvent fractions. We evaluated the potential cytotoxicity of these root extract fractions on Vero cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
We show that all solvent extract fractions (except methanolic solvent fractions) had cytotoxic concentration values that killed 50% of the Vero cells (CC) greater than 20 µg/mL and selectivity index (SI) greater than 1.0. Taken together, we demonstrate that, A. pluriseta extract fractions' earlier reported bioactivities are within the acceptable cytotoxicity and selective index limits. This finding scientifically validates the potential use of A. pluriseta in the discovery of safe therapeutics agents.
我们及其他研究人员已表明多裂阿斯皮利亚与多种生物活性相关。然而，关于其细胞毒性的信息却很匮乏。这造成了多裂阿斯皮利亚提取物安全性方面的信息空白。作为我们近期关于多裂阿斯皮利亚根提取物抗菌活性及植物化学特征研究成果的拓展，在此我们报告所测试溶剂提取物部分的细胞毒性。我们通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法评估了这些根提取物部分对非洲绿猴肾细胞系的潜在细胞毒性。
我们发现，所有溶剂提取物部分（甲醇溶剂提取物部分除外）的细胞毒性浓度值，即杀死50%非洲绿猴肾细胞的浓度（CC）大于20微克/毫升，且选择性指数（SI）大于1.0。综上所述，我们证明，多裂阿斯皮利亚提取物部分先前报道的生物活性处于可接受的细胞毒性和选择性指数范围内。这一发现从科学角度验证了多裂阿斯皮利亚在发现安全治疗药物方面的潜在用途。
