Department of Chemistry, University of Nairobi, P. O. Box 30197, 00100 Nairobi, Kenya.
Department of Chemistry, University of Jyvaskyla, P.O. Box 35, 40014 Jyvaskyla, Finland.
Molecules. 2018 Dec 4;23(12):3199. doi: 10.3390/molecules23123199.
A phytochemical investigation of the roots of led to the isolation of -kaurane-type diterpenoids and additional phytochemicals (⁻). The structures of the isolated compounds were elucidated based on Nuclear Magnetic Resonance (NMR) spectroscopic and mass spectrometric analyses. The absolute configurations of seven of the -kaurane-type diterpenoids (⁻, and ) were determined by single crystal X-ray diffraction studies. Eleven of the compounds were also isolated from the roots and the aerial parts of . The literature NMR assignments for compounds and were revised. In a cytotoxicity assay, 12α-methoxy--kaur-9(11),16-dien-19-oic acid () (IC = 27.3 ± 1.9 µM) and 9β-hydroxy-15α-angeloyloxy--kaur-16-en-19-oic acid () (IC = 24.7 ± 2.8 µM) were the most cytotoxic against the hepatocellular carcinoma (Hep-G2) cell line, while 15α-angeloyloxy-16β,17-epoxy--kauran-19-oic acid () (IC = 30.7 ± 1.7 µM) was the most cytotoxic against adenocarcinomic human alveolar basal epithelial (A549) cells.
对 led 的根部进行植物化学研究，分离得到了 -kaurane 型二萜类化合物和其他植物化学物质（⁻）。根据核磁共振（NMR）光谱和质谱分析确定了分离化合物的结构。通过单晶 X 射线衍射研究确定了七种 -kaurane 型二萜类化合物（⁻、和 ）的绝对构型。从根和地上部分也分离出了 11 种化合物。还修订了化合物 和 的文献 NMR 分配。在细胞毒性测定中，12α-甲氧基--kaur-9(11),16-二烯-19-酸（）（IC = 27.3 ± 1.9 µM）和 9β-羟基-15α-angeloyloxy--kaur-16-烯-19-酸（）（IC = 24.7 ± 2.8 µM）对肝癌（Hep-G2）细胞系的细胞毒性最强，而 15α-angeloyloxy-16β,17-epoxy--kauran-19-oic 酸（）（IC = 30.7 ± 1.7 µM）对腺癌细胞（A549）的细胞毒性最强。
