Richter Anja M, Buchberger Gerda, Stifter David, Duchoslav Jiri, Hertwig Andreas, Bonse Jörn, Heitz Johannes, Schwibbert Karin
Bundesanstalt für Materialforschung und -prüfung (BAM), Unter den Eichen 87, 12205 Berlin, Germany.
Institute of Applied Physics, Johannes Kepler University Linz, Altenberger Strasse 69, 4040 Linz, Austria.
Nanomaterials (Basel). 2021 Nov 8;11(11):3000. doi: 10.3390/nano11113000.
Bacterial adhesion and biofilm formation on surfaces are associated with persistent microbial contamination, biofouling, and the emergence of resistance, thus, calling for new strategies to impede bacterial surface colonization. Using ns-UV laser treatment (wavelength 248 nm and a pulse duration of 20 ns), laser-induced periodic surface structures (LIPSS) featuring different sub-micrometric periods ranging from 210 to ~610 nm were processed on commercial poly(ethylene terephthalate) (PET) foils. Bacterial adhesion tests revealed that these nanorippled surfaces exhibit a repellence for that decisively depends on the spatial periods of the LIPSS with the strongest reduction (91%) in cell adhesion observed for LIPSS periods of 214 nm. Although chemical and structural analyses indicated a moderate laser-induced surface oxidation, a significant influence on the bacterial adhesion was ruled out. Scanning electron microscopy and additional biofilm studies using a pili-deficient TG1 strain revealed the role of extracellular appendages in the bacterial repellence observed here.
细菌在表面的粘附和生物膜形成与持续的微生物污染、生物污垢以及耐药性的出现有关，因此需要新的策略来阻止细菌在表面定殖。使用纳秒紫外激光处理（波长248 nm，脉冲持续时间20 ns），在商用聚对苯二甲酸乙二酯（PET）箔上加工出具有不同亚微米周期（范围从210到610 nm）的激光诱导周期性表面结构（LIPSS）。细菌粘附测试表明，这些纳米波纹表面对[此处原文缺失具体指代对象]具有排斥性，这种排斥性决定性地取决于LIPSS的空间周期，对于214 nm的LIPSS周期，观察到细胞粘附减少最为显著（约91%）。尽管化学和结构分析表明存在适度的激光诱导表面氧化，但排除了其对细菌粘附的显著影响。扫描电子显微镜以及使用菌毛缺陷型TG1菌株进行的额外生物膜研究揭示了细胞外附属物在此处观察到的细菌排斥中的作用。
