Yin Rui, Yi Yan-Jun, Chen Zhuo, Chen Guan-Jun, Zhou Yan-Xia, Du Zong-Jun
Marine College, Shandong University, Weihai 264209, PR China.
Int J Syst Evol Microbiol. 2019 Jun;71(3). doi: 10.1099/ijsem.0.004701. Epub 2021 Feb 10.
A Gram-stain-negative, aerobic, yellow, non-motile, rod-shaped and alginate-degrading bacterium, designated Dm15, was isolated from marine alga collected in Weihai, PR China. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Dm15 represents a distinct line of the family . Strain Dm15 had the highest 16S rRNA gene sequence similarity to its closest phylogenetic neighbour (96.7 %) and 93.7-96.4 % sequence similarity to other phylogenetic neighbours (, , , and ) in the family . The novel isolate was able to grow at 10-40 °C (optimum, 30-33 °C), pH 7.0-9.0 (optimum, pH 7.0-7.5) and with 0.5-6.0 % NaCl (optimum 2.0-3.0 %, w/v). It could grow at 40 °C, and degrade alginate and cellulose, which were different from the neighbour genera. The draft genome consisted of 3395 genes with a total length of 3 798 431 bp and 34.1mol% G+C content. Especially, there were some specific genes coding for cellulase and alginate lyase, which provided a basis for the above phenotypic characteristics. The strain's genome sequence showed 71.1-80.2 % average amino acid identity values and 71.8-77.7 % average nucleotide identity values compared to the type strains of related genera within the family . It shared digital DNA-DNA hybridization identity of 19.8 and 20.9 % with and , respectively. The sole menaquinone was MK-6. The major fatty acids were iso-C and iso-C G. The polar lipids included six unidentified polar lipids, four unidentified aminolipids and phosphatidylethanolamine. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain Dm15 represents a novel species of a new genus in the family , phylum , for which the name gen. nov., sp. nov. is proposed. The type strain is Dm15 (KCTC 42256=CICC 23815).
从中国威海采集的海藻中分离出一株革兰氏阴性、需氧、黄色、无运动性、杆状且能降解藻酸盐的细菌，命名为Dm15。基于16S rRNA基因序列的系统发育分析表明，菌株Dm15代表了该科的一个独特谱系。菌株Dm15与其最接近的系统发育邻居的16S rRNA基因序列相似性最高（96.7%），与该科其他系统发育邻居（、、、和）的序列相似性为93.7 - 96.4%。该新分离菌株能够在10 - 40°C（最适温度为30 - 33°C）、pH 7.0 - 9.0（最适pH为7.0 - 7.5）以及0.5 - 6.0%（最适为2.0 - 3.0%，w/v）的NaCl条件下生长。它能够在40°C生长，并降解藻酸盐和纤维素，这与相邻属不同。草图基因组由3395个基因组成，总长度为3798431 bp，G + C含量为34.1mol%。特别地，存在一些编码纤维素酶和藻酸盐裂解酶的特定基因，这为上述表型特征提供了基础。与该科相关属的模式菌株相比，该菌株的基因组序列显示平均氨基酸同一性值为71.1 - 80.2%，平均核苷酸同一性值为71.8 - 77.7%。它与和的数字DNA - DNA杂交同一性分别为19.8%和20.9%。唯一的甲基萘醌是MK - 6。主要脂肪酸为异-C和异-C G。极性脂质包括六种未鉴定的极性脂质、四种未鉴定的氨基脂质和磷脂酰乙醇胺。基于表型、化学分类和系统发育分析结果，菌株Dm15代表了该科、门中一个新属的新物种，为此提出新属名，新种名。模式菌株为Dm15（KCTC 42256 = CICC 23815）。
