Dees S B, Moss C W, Hollis D G, Weaver R E
J Clin Microbiol. 1986 Feb;23(2):267-73. doi: 10.1128/jcm.23.2.267-273.1986.
The cellular fatty acid, sphingolipid, and isoprenoid quinone compositions of Flavobacterium odoratum, Flavobacterium breve, and Flavobacterium-like groups IIe, IIh, and IIf were determined, using thin-layer, gas-liquid, and reverse-phase high-performance liquid chromatography. The fatty acid data showed that groups IIe, IIh, and IIf were similar to recognized Flavobacterium species by the presence of relatively large amounts of iso-branched hydroxy and nonhydroxy acids. Groups IIe and IIh were essentially identical in fatty acid composition but were distinguished from group IIf, F. breve, and F. odoratum on the basis of minor qualitative and quantitative differences. All strains tested contained menaquinone 6 as the major isoprenoid quinone, and all lacked sphingolipids. Overall, the chemical data suggest that groups IIe, IIh, and IIf are additional Flavobacterium species and are different from sphingobacteria, which contain sphingolipid and menaquinone 7 as the major quinone.
利用薄层色谱、气液色谱和反相高效液相色谱法,测定了芳香黄杆菌、短黄杆菌以及类黄杆菌群IIe、IIh和IIf的细胞脂肪酸、鞘脂和类异戊二烯醌组成。脂肪酸数据表明,IIe、IIh和IIf群与公认的黄杆菌属物种相似,因为它们含有相对大量的异支链羟基酸和非羟基酸。IIe群和IIh群在脂肪酸组成上基本相同,但根据微小的定性和定量差异与IIf群、短黄杆菌和芳香黄杆菌区分开来。所有测试菌株均以甲基萘醌6作为主要类异戊二烯醌,且均缺乏鞘脂。总体而言,化学数据表明,IIe、IIh和IIf群是另外的黄杆菌属物种,与以鞘脂和甲基萘醌7作为主要醌的鞘氨醇杆菌不同。