Hudson M J, Hollis D G, Weaver R E, Galvis C G
Department of Medical Technology, University of Southern Mississippi, Hattiesburg 39406.
J Clin Microbiol. 1987 Oct;25(10):1907-10. doi: 10.1128/jcm.25.10.1907-1910.1987.
Thirty strains of microorganisms previously classified in the CDC group EO-2 were studied to determine the relationship of this group and the recently proposed Psychrobacter immobilis. Results of transformation studies indicated that 9 of the 30 studied strains should be reclassified as P. immobilis. While transformation studies should be used to identify the Psychrobacter genus definitively, some presumptive characteristics useful in differentiating the CDC group EO-2 and P. immobilis were observed. The Psychrobacter genus, compared with the CDC group EO-2, grew well at 25 degrees C, lightly or not at all at 35 degrees C, and not at all at 42 degrees C, grew in nutrient broth without NaCl and with only one exception grew in 6% NaCl, usually did not produce acid from adonitol, dextrin, fructose, or D-mannitol, did not demonstrate the O-shaped cellular morphology, and usually possessed an odor resembling that noted from a phenylethyl alcohol blood agar plate.
对先前归类于疾病控制与预防中心(CDC)EO-2组的30株微生物进行了研究,以确定该组与最近提出的不动冷杆菌(Psychrobacter immobilis)之间的关系。转化研究结果表明,所研究的30株菌株中有9株应重新归类为不动冷杆菌。虽然转化研究应用于明确鉴定冷杆菌属,但也观察到了一些有助于区分CDC EO-2组和不动冷杆菌的推定特征。与CDC EO-2组相比,冷杆菌属在25℃下生长良好,在35℃下生长微弱或根本不生长,在42℃下完全不生长,能在不含氯化钠的营养肉汤中生长,除一个例外,在6%氯化钠中也能生长,通常不能从阿东糖醇、糊精、果糖或D-甘露醇产酸,不呈现O形细胞形态,并且通常具有类似于苯乙醇血琼脂平板上所闻到的气味。