Milani Paula Gimenez, Piovan Silvano, Lima Yago Carvalho, Zorzenon Maria Rosa Trentin, da Rosa Carlos Vinicius Dalto, Peixoto Giuliana Maria Ledesma, de Freitas Mathias Paulo Cezar, Natali Maria Raquel Marçal, da Costa Silvio Claudio, Mareze-Costa Cecília Edna
Department of Biochemistry, State University of Maringá, Maringá, PR Brazil.
Department of Biotechnology, Genetics and Cell Biology, State University of Maringá, Maringá, PR Brazil.
J Food Sci Technol. 2021 Feb;58(2):805-810. doi: 10.1007/s13197-020-04799-3. Epub 2020 Sep 25.
The study aims to analyse the treatment of whey protein enriched with fraction in insulin secretion and its role mitigating streptozotocin-induced hyperglycemia in rats. Thus, diabetic animals were treated with whey protein enriched with fraction or with only the protein isolate or only the Stevia fraction. Insulin level in plasma was measured by radioimmunoassay and the viability of β cells was detected by immunohistochemistry. The results showed that diabetic animals treated with whey protein enriched with fraction had a greater recovery from insulinemia, with plasma levels similar to non-diabetic animals (~ 0.13 ng/mL). In addition, the same group showed a higher number of insulin-positive pancreatic B cells (~ 66%) in immunohistochemistry analysis, while the diabetic groups treated with only the fraction of stevia or whey protein showed 38 and 59% of positive cells, respectively. These results show that the treatment may have restored the viability of streptozotocin-injured pancreatic B cells, and consequently increased insulin secretion, suggesting whey protein enriched with fraction can be used an adjunct/supplement in diabetic treatment.
该研究旨在分析富含 组分的乳清蛋白在胰岛素分泌中的治疗作用及其减轻链脲佐菌素诱导的大鼠高血糖的作用。因此，对糖尿病动物用富含 组分的乳清蛋白或仅用蛋白质分离物或仅用甜菊糖苷组分进行治疗。通过放射免疫测定法测量血浆中的胰岛素水平，并通过免疫组织化学检测β细胞的活力。结果表明，用富含 组分的乳清蛋白治疗的糖尿病动物胰岛素血症恢复得更好，血浆水平与非糖尿病动物相似（约0.13 ng/mL）。此外，在免疫组织化学分析中，同一组显示胰岛素阳性胰腺B细胞数量更多（约66%），而仅用甜菊糖苷组分或乳清蛋白治疗的糖尿病组分别显示38%和59%的阳性细胞。这些结果表明，该治疗可能恢复了链脲佐菌素损伤的胰腺B细胞的活力，从而增加了胰岛素分泌，提示富含 组分的乳清蛋白可作为糖尿病治疗的辅助/补充剂。
