Lee W, Burnie J P
Department of Medical Microbiology, St Bartholomew's Hospital, West Smithfield, London.
J Med Microbiol. 1988 Apr;25(4):261-8. doi: 10.1099/00222615-25-4-261.
A series of 133 isolates of methicillin-resistant Staphylococcus aureus was fingerprinted by the immunoblot technique. Extracts were prepared by lysostaphin degradation of overnight cultures and peptides were separated by SDS polyacrylamide gel electrophoresis. The peptides were transblotted on to nitrocellulose membranes and probed with (1) a hyperimmune rabbit serum raised against a methicillin-resistant S. aureus isolate, (2) a hyperimmune rabbit serum raised against an isolate of S. epidermidis, and (3) serum from a patient who had recovered from an infection with a methicillin-resistant S. aureus. This typing method confirmed the existence of an epidemic strain that accounted for 102 of the isolates. The remaining 31 isolates were grouped into a further seven types which correlated with the results of phage typing and antibiograms.
采用免疫印迹技术对133株耐甲氧西林金黄色葡萄球菌分离株进行指纹图谱分析。通过溶葡萄球菌素降解过夜培养物制备提取物，并用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离肽段。将肽段转印到硝酸纤维素膜上，并用以下试剂进行检测：(1) 针对耐甲氧西林金黄色葡萄球菌分离株制备的超免疫兔血清；(2) 针对表皮葡萄球菌分离株制备的超免疫兔血清；(3) 一名从耐甲氧西林金黄色葡萄球菌感染中康复的患者的血清。这种分型方法证实存在一种流行菌株，该菌株占分离株的102株。其余31株分离株进一步分为另外七种类型，这与噬菌体分型和抗菌谱结果相关。
