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通过荧光显微镜对使用旋转和往复系统进行根管预备后的根管内活力评估。

Intratubular viability assessment following root canal instrumentation with rotary and reciprocating systems via fluorescence microscopy.

作者信息

Ghoddusi Jamileh, Arian Ehsan, Golmohammadi Maryam, Gharechahi Maryam, Moushekhian Siavash

机构信息

Dental Research Center, Faculty of Dentistry, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran.

Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

出版信息

J Dent Res Dent Clin Dent Prospects. 2020 Fall;14(4):214-217. doi: 10.34172/joddd.2020.044. Epub 2020 Dec 2.

DOI:10.34172/joddd.2020.044
PMID:33575009
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7867686/
Abstract

The present in vitro study aimed to compare the effectiveness of the WaveOne and ProTaper Gold systems in removing the biofilm. Thirty-eight mandibular premolars were selected. The root canals were assigned to standard control (canals serially enlarged with ProTaper Gold S1-S2-F1-F2, n=15) and experimental (canals enlarged with Primary WaveOne file, n=15) groups. Following the instrumentation procedure, the root canals underwent a sampling procedure, and the colonyforming unit (CFU) counts were determined. The samples were also evaluated under a fluorescent microscope to evaluate viable bacteria. The data were analyzed using independent samples t test and paired samples test. The results showed that, compared with the ProTaper group, the WaveOne group exhibited the least viable bacteria ( =0.004). It was concluded that comparison with the ProTaper Gold rotary system, the WaveOne reciprocating file is more successful in reducing intratubular viable bacteria counts.

摘要

本体外研究旨在比较WaveOne系统和ProTaper Gold系统在清除生物膜方面的有效性。选取了38颗下颌前磨牙。将根管分为标准对照组(用ProTaper Gold S1-S2-F1-F2依次扩大根管,n = 15)和实验组(用WaveOne初尖锉扩大根管,n = 15)。在进行根管预备后,对根管进行采样,并测定菌落形成单位(CFU)计数。还在荧光显微镜下对样本进行评估以评估活菌。使用独立样本t检验和配对样本检验对数据进行分析。结果显示,与ProTaper组相比,WaveOne组的活菌最少(P = 0.004)。得出的结论是,与ProTaper Gold旋转系统相比,WaveOne往复锉在减少根管内活菌计数方面更成功。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/726d/7867686/f86f8be5f4e8/joddd-14-214-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/726d/7867686/3afd2a24d11c/joddd-14-214-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/726d/7867686/f86f8be5f4e8/joddd-14-214-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/726d/7867686/3afd2a24d11c/joddd-14-214-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/726d/7867686/f86f8be5f4e8/joddd-14-214-g002.jpg

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本文引用的文献

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2
Endodontic retreatment: clinical comparison of reciprocating systems versus rotary system in disinfecting root canals.根管再治疗:往复式系统与旋转式系统在根管消毒中的临床比较
Clin Oral Investig. 2015 Jul;19(6):1411-7. doi: 10.1007/s00784-014-1360-9. Epub 2014 Nov 21.
3
Viability and antibacterial efficacy of four root canal disinfection techniques evaluated using confocal laser scanning microscopy.
使用共聚焦激光扫描显微镜评估四种根管消毒技术的生存能力和抗菌效果。
J Conserv Dent. 2014 Sep;17(5):444-8. doi: 10.4103/0972-0707.139833.
4
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J Endod. 2014 May;40(5):625-9. doi: 10.1016/j.joen.2013.12.006. Epub 2014 Feb 2.
5
The Effect of Size and Taper of Apical Preparation in Reducing Intra-Canal Bacteria: A Quantitative SEM Study.根尖预备的尺寸和锥度对减少根管内细菌的影响:一项定量扫描电子显微镜研究
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