Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran; Cellular and Molecular Research Centre, Iran University of Medical Sciences, Tehran, Iran; Department of Textile Engineering, Amirkabir University of Technology, Tehran, Iran; Department of Pharmaceutics, University of Minnesota, MN, USA.
Department of Textile Engineering, Amirkabir University of Technology, Tehran, Iran.
Mater Sci Eng C Mater Biol Appl. 2021 Feb;121:111855. doi: 10.1016/j.msec.2020.111855. Epub 2021 Jan 6.
Mechanical properties of tissue engineering nanofibrous scaffolds are of importance because they not only determine their ease of application, but also influence the environment for cell growth and proliferation. Cellulose nanocrystals (CNCs) are natural renewable nanoparticles that have been widely used for manipulating nanofibers' mechanical properties. In this article, cellulose nanoparticles were incorporated into poly(caprolactone) (PCL) solution, and composite nanofibers were produced. Ozawa-Flynn-Wall (OFW) methodology and X-ray diffraction were used to investigate the effect of CNC incorporation on PCL crystalline structure and its biological properties. Results showed that CNC incorporation up to 1% increases the crystallization activation energy and reduces the crystal volume, while these factors remain constant above this critical concentration. MTT assay and microscopic images of seeded cells on the nanofiber scaffolds indicated increased cell growth on the samples containing CNC. This behavior could be attributed to their greater hydrophilicity, which was confirmed using parallel exponential kinetics (PEK) model fitting to results obtained from dynamic vapor sorption (DVS) studies. Superior performance of CNC containing samples was also confirmed by in vivo implantation on full-thickness wounds. The wound area faded away more rapidly in these samples. H&E and Masson's trichrome staining showed better regeneration and more developed tissues in wounds treated with PCL-CNC1% nanofibers.
组织工程纳米纤维支架的力学性能很重要，因为它们不仅决定了其应用的便利性，而且还影响了细胞生长和增殖的环境。纤维素纳米晶体（CNC）是天然可再生的纳米颗粒，已被广泛用于控制纳米纤维的力学性能。本文将纤维素纳米颗粒掺入聚己内酯（PCL）溶液中，制备出复合纳米纤维。采用 Ozawa-Flynn-Wall（OFW）方法和 X 射线衍射研究了 CNC 掺入对 PCL 晶体结构及其生物性能的影响。结果表明，CNC 掺入量达到 1%时，会增加 PCL 的结晶活化能并减少晶体体积，而在超过这一临界浓度时，这些因素保持不变。MTT 试验和细胞在纳米纤维支架上的显微镜图像表明，含有 CNC 的样品上的细胞生长增加。这种行为可以归因于它们更高的亲水性，这通过对动态蒸汽吸附（DVS）研究结果进行平行指数动力学（PEK）模型拟合得到了证实。体内全层伤口植入实验也证实了含有 CNC 的样品具有更好的性能。这些样品的伤口面积更快地消失。H&E 和 Masson 三色染色显示，用 PCL-CNC1%纳米纤维处理的伤口有更好的再生和更发达的组织。
