Hivechi Ahmad, Milan Peiman Brouki, Modabberi Khashayar, Amoupour Moein, Ebrahimzadeh Kaveh, Gholipour Amir Reza, Sedighi Faezeh, Amini Naser, Bahrami S Hajir, Rezapour Alireza, Hamidi Masoud, Delattre Cédric
Department of Textile Engineering, School of Materials and Advanced Processing, Amirkabir University of Technology, Tehran 1591639675, Iran.
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran 1591639675, Iran.
Polymers (Basel). 2021 Mar 10;13(6):854. doi: 10.3390/polym13060854.
Loss of skin integrity can lead to serious problems and even death. In this study, for the first time, the effect of exopolysaccharide (EPS) produced by cold-adapted yeast sp. GUMS16 on a full-thickness wound in rats was evaluated. The GUMS16 strain's EPS was precipitated by adding cold ethanol and then lyophilized. Afterward, the EPS with polycaprolactone (PCL) and gelatin was fabricated into nanofibers with two single-needle and double-needle procedures. The rats' full-thickness wounds were treated with nanofibers and Hematoxylin and eosin (H&E) and Masson's Trichrome staining was done for studying the wound healing in rats. Obtained results from SEM, DLS, FTIR, and TGA showed that EPS has a carbohydrate chemical structure with an average diameter of 40 nm. Cell viability assessments showed that the 2% EPS loaded sample exhibits the highest cell activity. Moreover, in vivo implantation of nanofiber webs on the full-thickness wound on rat models displayed a faster healing rate when EPS was loaded into a nanofiber. These results suggest that the produced EPS can be used for skin tissue engineering applications.
皮肤完整性丧失会导致严重问题甚至死亡。在本研究中，首次评估了冷适应酵母菌株GUMS16产生的胞外多糖（EPS）对大鼠全层伤口的影响。通过添加冷乙醇沉淀GUMS16菌株的EPS，然后冻干。之后，将EPS与聚己内酯（PCL）和明胶通过单针和双针两种工艺制成纳米纤维。用纳米纤维处理大鼠的全层伤口，并进行苏木精和伊红（H&E）染色以及Masson三色染色，以研究大鼠的伤口愈合情况。扫描电子显微镜（SEM）、动态光散射（DLS）、傅里叶变换红外光谱（FTIR）和热重分析（TGA）获得的结果表明，EPS具有碳水化合物化学结构，平均直径为40纳米。细胞活力评估表明，负载2% EPS的样品表现出最高的细胞活性。此外，当将EPS负载到纳米纤维中时，在大鼠模型的全层伤口上体内植入纳米纤维网显示出更快的愈合速度。这些结果表明，所产生的EPS可用于皮肤组织工程应用。
