Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, 9301, South Africa.
Department of Animal Wildlife and Grassland Sciences, University of the Free State, Bloemfontein, 9301, South Africa.
G3 (Bethesda). 2021 Apr 15;11(4). doi: 10.1093/g3journal/jkab042.
Candida albicans is frequently co-isolated with the Gram-negative bacterium, Pseudomonas aeruginosa. In vitro, the interaction is complex, with both species influencing each other. Not only does the bacterium kill hyphal cells of C. albicans through physical interaction, it also affects C. albicans biofilm formation and morphogenesis, through various secreted factors and cell wall components. The present study sought to expand the current knowledge regarding the interaction between C. albicans and P. aeruginosa, using transcriptome analyses of early static biofilms. Under these conditions, a total of 2,537 open reading frames (approximately 40% of the C. albicans transcriptome) was differentially regulated in the presence of P. aeruginosa. Upon deeper analyses it became evident that the response of C. albicans toward P. aeruginosa was dominated by a response to hypoxia, and included those associated with stress as well as iron and zinc homeostasis. These conditions may also lead to the observed differential regulation of genes associated with cell membrane synthesis, morphology, biofilm formation and phenotypic switching. Thus, C. albicans in polymicrobial biofilms with P. aeruginosa have unique transcriptional profiles that may influence commensalism as well as pathogenesis.
白色念珠菌常与革兰氏阴性菌铜绿假单胞菌共同分离。在体外，两者的相互作用非常复杂，相互影响。这种细菌不仅通过物理相互作用杀死白色念珠菌的菌丝细胞，还通过各种分泌因子和细胞壁成分影响白色念珠菌生物膜的形成和形态发生。本研究旨在通过早期静态生物膜的转录组分析，扩展对白色念珠菌和铜绿假单胞菌相互作用的现有认识。在这些条件下，铜绿假单胞菌的存在共调节了 2537 个开放阅读框（约占白色念珠菌转录组的 40%）。进一步分析表明，白色念珠菌对铜绿假单胞菌的反应主要是对缺氧的反应，包括与应激以及铁和锌稳态相关的反应。这些条件也可能导致与细胞膜合成、形态、生物膜形成和表型转换相关的基因的差异调节。因此，在与铜绿假单胞菌形成的多微生物生物膜中的白色念珠菌具有独特的转录谱，这可能影响共生和发病机制。
