Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK.
Institute of Microbiology and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, UK.
Microbiology (Reading). 2023 Jan;169(1). doi: 10.1099/mic.0.001271.
is a Gram-negative opportunistic pathogen frequently isolated from chronic infections of the cystic fibrosis lung and burn wounds, and is a major cause of antimicrobial-resistant nosocomial infections. is frequently co-isolated with the opportunistic fungal pathogen with the presence of in dual-species biofilms promoting tolerance to meropenem. Here, transcription profiling of mature single- or dual-species biofilms was carried out to understand the molecular mechanism(s) by which enhances meropenem tolerance. appeared to have a mild impact on the transcriptome of mature biofilms, with most differentially regulated genes being involved in interkingdom interactions (i.e. quorum sensing and phenazine biosynthesis). The addition of meropenem to mature single- or dual-species biofilms resulted in a significant bacterial transcriptional response, including the induction of the beta-lactamase, , genes involved in biofilm formation. elicited a similar transcriptional response to meropenem in the presence of , but promoted the expression of additional efflux pumps, which could play roles in increasing the tolerance of to meropenem.
是一种革兰氏阴性机会性病原体，常从囊性纤维化肺和烧伤感染的慢性感染中分离出来，也是导致抗微生物药物耐药性医院感染的主要原因。 常与机会性真菌病原体 共同分离， 存在于双物种生物膜中，促进了对美罗培南的耐受性。在这里，对成熟的 单种或双种生物膜进行了转录谱分析，以了解 增强美罗培南耐受性的分子机制。 对成熟生物膜的转录组似乎有轻微的影响，大多数差异调节基因涉及种间相互作用（即群体感应和吩嗪生物合成）。将美罗培南添加到成熟的单种或双种生物膜中会导致细菌转录反应显著，包括诱导β-内酰胺酶 、参与生物膜形成的基因。 在 的存在下对美罗培南产生类似的转录反应，但 促进了其他外排泵的表达，这可能在增加 对美罗培南的耐受性方面发挥作用。
