National Institute of Public Health NIH - National Research Institute, Warsaw, 00-791, Poland.
Museum and Institute of Zoology, Polish Academy of Sciences, Twarda 51/55, Warsaw, 00-818, Poland.
Sci Rep. 2024 Nov 18;14(1):28436. doi: 10.1038/s41598-024-77295-9.
Borrelia miyamotoi spirochete is carried by Ixodidae ticks and causing Borrelia miyamotoi disease (BMD), a relapsing fever illness reported worldwide, often in the same geographic areas where Lyme disease is endemic. The purpose of this study was to examine the presence of B. miyamotoi infection in Dermacentor reticulatus and Ixodes ricinus ticks collected within the boundaries of the Chornobyl Exclusion Zone, Ukraine. A total of 1176 adult D. reticulatus ticks and 113 adult I. ricinus ticks sampled from five different habitats in the Chornobyl Exclusion Zone, Ukraine, were tested for Borrelia miyamotoi using a two-step nested PCR procedure. Borrelia miyamotoi was found in two (0.17%) out of the 1176 D. reticulatus ticks tested. The obtained sequences were submitted to GenBank (MZ365312-MZ365313). Phylogenetic analysis showed that the B. miyamotoi sequences detected cluster together with a strain originating from I. scapularis in the USA. The strain identified in the Chornobyl Exclusion Zone belongs to the group of strains found in Eastern Europe and Asia, north of the Himalayas. No I. ricinus ticks were found to be infected. This study presents the first detection of B. miyamotoi in D. reticulatus ticks in the Chornobyl Exclusion Zone, Ukraine.
伯氏疏螺旋体是由硬蜱属蜱传播的，引起伯氏疏螺旋体病（BMD），这是一种世界性反复发作的发热疾病，通常与莱姆病流行的地理区域相同。本研究的目的是检查乌克兰切尔诺贝利隔离区边界内采集的革螨和蓖子硬蜱中是否存在伯氏疏螺旋体感染。使用两步嵌套 PCR 程序对来自乌克兰切尔诺贝利隔离区五个不同生境的 1176 只成年革螨和 113 只成年蓖子硬蜱进行了伯氏疏螺旋体检测。在检测的 1176 只革螨中，有 2 只（0.17%）发现携带伯氏疏螺旋体。获得的序列已提交给 GenBank（MZ365312-MZ365313）。系统发育分析表明，检测到的伯氏疏螺旋体序列与来自美国的 I. scapularis 的菌株聚类在一起。在切尔诺贝利隔离区鉴定的菌株属于在东欧和亚洲、喜马拉雅山以北发现的菌株群。未发现蓖子硬蜱感染。本研究首次在乌克兰切尔诺贝利隔离区的革螨中检测到伯氏疏螺旋体。
