用于脑脊液和关节液标本常规培养的肉汤培养基评估。

Evaluation of broth media for routine culture of cerebrospinal and joint fluid specimens.

作者信息

Reinhold C E, Nickolai D J, Piccinini T E, Byford B A, York M K, Brooks G F

机构信息

Department of Laboratory Medicine, University of California, San Francisco 94143.

出版信息

Am J Clin Pathol. 1988 May;89(5):671-4. doi: 10.1093/ajcp/89.5.671.

DOI:10.1093/ajcp/89.5.671

PMID:3358372

Abstract

Broth cultures of cerebrospinal and joint fluids are important components in the culture detection of meningitis and septic arthritis. The authors examined 121 strains of bacteria isolated from clinical specimens representing 13 species or groups that cause meningitis and arthritis for growth in supplemented Thioglycolate broth (THIO), Supplemented Peptone Broth (SPB), and minced beef heart (MBH) media each alone or with added IsoVitaleX. Both SPB and MBH with IsoVitaleX performed better as broth culture media than the media without IsoVitaleX or THIO with or without IsoVitaleX.

摘要

脑脊液和关节液的肉汤培养物是脑膜炎和脓毒性关节炎培养检测中的重要组成部分。作者检测了从临床标本中分离出的121株细菌，这些细菌代表了13种导致脑膜炎和关节炎的菌种或菌群，观察它们在添加了硫乙醇酸盐肉汤（THIO）、蛋白胨肉汤（SPB）和碎牛心（MBH）培养基中单独培养或添加异维生素（IsoVitaleX）后的生长情况。添加了IsoVitaleX的SPB和MBH作为肉汤培养基，比未添加IsoVitaleX的培养基或添加或未添加IsoVitaleX的THIO表现更好。