Silletti R P, Ailey E, Sun S, Tang D
Division of Clinical Microbiology, Elmhurst Hospital Center, New York 11373, USA.
J Clin Microbiol. 1997 Aug;35(8):2003-6. doi: 10.1128/jcm.35.8.2003-2006.1997.
In order to assess the microbiologic and clinical value of primary broth culture of wound specimens collected with swabs and submitted to the laboratory in transport medium, we compared the results of primary agar culture with the results of a corresponding primary broth culture for 344 aerobic specimens and 176 anaerobic specimens. While 8.7% (45 of 520) of the specimens yielded organisms from the primary broth culture that were not recovered from the corresponding primary agar culture, only 5.0% (26 of 520) of the specimens yielded organisms from the primary broth culture other than Staphylococcus epidermidis, viridans group streptococci, and Corynebacterium spp. Moreover, the primary broth culture of only 0.6% (3 of 520) of the specimens yielded organisms not recovered from the primary agar culture that caused a change in the therapy of the patient. Our conclusion is that primary broth cultures are unnecessary for the processing of wound specimens properly collected with swabs.
为了评估用拭子采集并置于运送培养基中送检至实验室的伤口标本的初代肉汤培养的微生物学和临床价值，我们比较了344份需氧标本和176份厌氧标本的初代琼脂培养结果与相应的初代肉汤培养结果。虽然8.7%（520份中的45份）的标本从初代肉汤培养中培养出了在相应的初代琼脂培养中未培养出的微生物，但只有5.0%（520份中的26份）的标本从初代肉汤培养中培养出了除表皮葡萄球菌、草绿色链球菌和棒状杆菌属之外的微生物。此外，只有0.6%（520份中的3份）的标本的初代肉汤培养培养出了在初代琼脂培养中未培养出且导致患者治疗方案改变的微生物。我们的结论是，对于用拭子正确采集的伤口标本，初代肉汤培养并无必要。
