Yang Fengyuan, Zhao Shanshan, Wang Yuan, Fan Xiaomiao, Wang Yanping, Feng Changsong
Henan Provincial Key Laboratory of Ion Beam Bio-engineering, School of Agricultural Science, Zhengzhou University, Zhengzhou, China.
Henan Provincial Key Laboratory of Ion Beam Bio-engineering, College of Physics, Zhengzhou University, Zhengzhou, China.
Front Microbiol. 2021 Jan 26;11:629894. doi: 10.3389/fmicb.2020.629894. eCollection 2020.
Relative quantification 16S-seq (RQS) has drawn deeper insights into bacterial community compositions in silage. However, it provides no information on dynamics of the total amount of bacterial DNA through the ensiling process and across different treatments. In this study, bacterial compositions in alfalfa silage with and without inoculation after 10 and 60days of ensiling were investigated using absolute quantification 16S-seq (AQS), and bacterial composition and its interaction with fermentation properties of silage indicated by AQS and RQS were compared. Variation in total bacterial DNA amounts across different treatments and ensiling periods was illustrated by AQS. AQS indicated higher bacterial richness indices and closer correlations of these indices with fermentation properties than RQS spearman's correlation analyses, as well as more taxa with significance on bacterial abundance lefse analyses. In conclusion, AQS effectively illustrated the dynamics of bacterial communities during the ensiling process.
相对定量16S测序（RQS）对青贮饲料中的细菌群落组成有了更深入的了解。然而，它没有提供关于青贮过程中以及不同处理方式下细菌DNA总量动态变化的信息。在本研究中，使用绝对定量16S测序（AQS）研究了苜蓿青贮饲料在青贮10天和60天后接种和未接种情况下的细菌组成，并比较了AQS和RQS所表明的细菌组成及其与青贮饲料发酵特性的相互作用。AQS说明了不同处理和青贮时期总细菌DNA量的变化。AQS显示出比RQS更高的细菌丰富度指数，并且这些指数与发酵特性的相关性更紧密（斯皮尔曼相关性分析），以及在细菌丰度上具有显著意义的更多分类群（线性判别分析效应大小法分析）。总之，AQS有效地说明了青贮过程中细菌群落的动态变化。
