Environment Biomonitoring Laboratory (LR01/ES14), Sciences Faculty of Bizerte, University of Carthage, Zarzouna, Tunisia.
Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome, Laboratory affiliated to Istituto Pasteur Italia-Fondazione Cenci Bolognetti, Latina, Italy.
Biofouling. 2021 Feb;37(2):174-183. doi: 10.1080/08927014.2021.1886278. Epub 2021 Feb 15.
Enteropathogenic E2346/69 (EPEC) has caused foodborne outbreaks worldwide and the bacterium forms antimicrobial-tolerant biofilms. The anti-biofilm formation of various components of essential oils extracted from selected medicinal plants were investigated and tested on EPEC and wild strains of . Oils extracted from the family Asteraceae and their major common constituents at 0.031 and 0.062% (V/v) were found to significantly inhibit biofilm formation without affecting the growth of planktonic cells. In addition, three plants belonging to this family (, and ) played important roles in the antimicrobial activity. Interestingly, their essential oils reduced the ability of . (the EPEC and K12 strains) to form a biofilm. The crystal violet reduction assay showed that the plant extracts tested reduced biofilm formation with the inhibition of bacterial attachment up to 45% for EPEC and 70% for K-12 after 24 h treatment at 0.62 mg ml, demonstrating that oils had a high anti-biofilm activity on the bacteria tested. The results indicate that the locus of enterocyte effacement (LEE) acquired by horizontal transfer promotes the formation of the attaching and effacing (A/E) lesion and increases the capacity of the photogen strain (EPEC) to form a biofilm. The chemical composition of the volatile compounds was obtained by gas chromatography-mass spectrometry analysis, which showed that the essential oils consisted of thirty-four compounds. Chamazulene (39.21%), β-pinene (32.07%), and α-thujone (29.39%) were the main constituents of the essential oils of , and respectively.
肠致病性大肠杆菌 E2346/69（EPEC）已在全球范围内引发食源性疾病爆发，并且该细菌会形成具有抗药性的生物膜。本研究调查了从选定药用植物中提取的各种精油成分的抗生物膜形成作用，并在 EPEC 和野生菌株上进行了测试。从菊科植物中提取的油及其主要共同成分在 0.031 和 0.062%（V/v）时，被发现可显著抑制生物膜形成，而不会影响浮游细胞的生长。此外，该科的三种植物（、和）在抗菌活性中发挥了重要作用。有趣的是，它们的精油降低了. （EPEC 和 K12 菌株）形成生物膜的能力。结晶紫还原测定表明，在 0.62 mg ml 下处理 24 小时后，测试的植物提取物可减少生物膜形成，对 EPEC 的抑制率达到 45%，对 K-12 的抑制率达到 70%，表明植物精油对测试的细菌具有很高的抗生物膜活性。结果表明，水平转移获得的肠细胞黏附素（LEE）基因座促进附着和消蚀（A/E）病变的形成，并增加了光产菌株（EPEC）形成生物膜的能力。通过气相色谱-质谱分析获得了挥发性化合物的化学组成，结果表明精油由三十四种化合物组成。天蓝烃（39.21%）、β-蒎烯（32.07%）和α-侧柏酮（29.39%）分别是、和精油的主要成分。
