Liu Feifei, Wang Xiaohui, Chen Tongtong, Zhang Naiyin, Wei Qin, Tian Juling, Wang Yingbo, Ma Chuang, Lu Yong
College of Chemical Engineering, Xinjiang Normal University, Urumqi 830054, Xinjiang, PR China.
Radiology Department, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai 200025, PR China.
J Adv Res. 2019 Oct 9;21:91-102. doi: 10.1016/j.jare.2019.10.002. eCollection 2020 Jan.
Bone implant materials cause the most common complication of bone infections in orthopedic surgery, resulting in implant failure. Antibiotic treatment of bone infections leads to problems such as bacterial resistance and reduced osteogenic capacity. In this study, dopamine (DA) was self-polymerized on the surface of Polylactic acid (PLLA)/Hydroxyapatite (HA) nanowire composite fibers to form an adhesive polydopamine (PDA) membrane, and a stable silver-nanoparticles (Ag-NPs) coating layer was constructed on it by electrochemically driven Ag coordination and chelation through Polypyrrole (PPy) mediation, achieving steady and slow release of Ag-NPs. With optimized DA soaking time of 24 h and soaking concentration of 0.5 g·L, nanoparticles were uniformly distributed on PLLA/HA/PDA/PPy/Ag composite fibers and the hydrophilicity of the composite fibers was well-behaved. Besides, the composite fibers possessed good physiological stability and 100% antibacterial rate against () as well as (). In addition, the composite fibers had promoted apatite nucleation and growth on surface and good cytocompatibility with osteoblasts, indicating ability of inducing osteogenic differentiation. In summary, a multi-functional PLLA/HA/PDA/PPy/Ag composite fiber with long-term antibacterial property, bioactivity and osteoinductivity was successfully constructed by electrospinning and electrochemical deposition.
骨植入材料是骨科手术中导致骨感染最常见的并发症，会导致植入物失效。骨感染的抗生素治疗会引发细菌耐药性和骨生成能力降低等问题。在本研究中，多巴胺（DA）在聚乳酸（PLLA）/羟基磷灰石（HA）纳米线复合纤维表面自聚合形成粘性聚多巴胺（PDA）膜，并通过聚吡咯（PPy）介导的电化学驱动银配位和螯合作用在其上构建稳定的银纳米颗粒（Ag-NPs）涂层，实现Ag-NPs的稳定缓释。在DA浸泡时间优化为24小时、浸泡浓度为0.5 g·L时，纳米颗粒均匀分布在PLLA/HA/PDA/PPy/Ag复合纤维上，且复合纤维的亲水性良好。此外，复合纤维具有良好的生理稳定性，对（）和（）的抗菌率达100%。另外，复合纤维促进了表面磷灰石的成核和生长，与成骨细胞具有良好的细胞相容性，表明其具有诱导成骨分化的能力。综上所述，通过静电纺丝和电化学沉积成功构建了一种具有长期抗菌性能、生物活性和骨诱导性的多功能PLLA/HA/PDA/PPy/Ag复合纤维。
