Li Shanshan, Zheng Meng, Zhang Zhentang, Peng Hengying, Dai Wenling, Liu Jihua
Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 211198, People's Republic of China.
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 211198, People's Republic of China.
Chin Med. 2021 Feb 16;16(1):22. doi: 10.1186/s13020-021-00432-3.
Galli gigeriae endothelium corneum (GGEC) has been effectively used for centuries for the treatment of functional dyspepsia (FD) in clinical practice in Asian countries. However, its potential mechanism and chemical composition remains undertermined.
In this study, the chemical profile of GGEC ethyl acetate extract (EAE) was evaluated by HPLC-Q-TOF-MS/MS. The effects of EAE on intestinal barrier function and inflammation were investigated in IEC-6 cells and RAW264.7 cells.
The results showed that 33 compounds were tentatively identified, including 12 soy isoflavones, 7 bile acids for the first time in EAE. EAE significantly reinforced intestinal barrier function via increasing the tight junction protein levels of ZO-1 and Occludin, reducing the mRNA expression levels of interleukin (IL)-1β and IL-6 in tumor necrosis factor alpha (TNF-α)-challenged IEC-6 cells. The scratch wound assay showed that EAE accelerated wound healing of IEC-6 cells. EAE evidently reduced the level of NO in a dose-dependent manner with an IC value of 18.12 μg/mL, and the mRNA expression of TNF-α, IL-1β, IL-6, iNOS and COX-2 in LPS-treated RAW264.7 cells.
This study revealed the intestinal barrier protective effects and chemical profile of GGEC, and the results indicated that GGEC strengthened the intestinal barrier by up-regulating protein expression of tight junctions and limiting inflammatory responses.
几个世纪以来，亚洲国家在临床实践中，鸡内金内皮角质层（GGEC）已被有效地用于治疗功能性消化不良（FD）。然而，其潜在机制和化学成分仍未确定。
在本研究中，采用高效液相色谱-四极杆飞行时间串联质谱（HPLC-Q-TOF-MS/MS）对GGEC乙酸乙酯提取物（EAE）的化学特征进行了评估。在IEC-6细胞和RAW264.7细胞中研究了EAE对肠道屏障功能和炎症的影响。
结果表明，初步鉴定出33种化合物，其中包括12种大豆异黄酮，7种胆汁酸首次在EAE中被发现。EAE通过增加紧密连接蛋白ZO-1和闭合蛋白的水平，降低肿瘤坏死因子-α（TNF-α）刺激的IEC-6细胞中白细胞介素（IL）-1β和IL-6的mRNA表达水平，显著增强肠道屏障功能。划痕试验表明，EAE加速了IEC-6细胞的伤口愈合。EAE以剂量依赖性方式明显降低一氧化氮（NO）水平，IC50值为18.12μg/mL，并降低脂多糖（LPS）处理的RAW264.7细胞中TNF-α、IL-1β、IL-6、诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）的mRNA表达。
本研究揭示了GGEC的肠道屏障保护作用和化学特征，结果表明GGEC通过上调紧密连接蛋白表达和限制炎症反应来增强肠道屏障。
