Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Health and Food Science Precinct, Kessels Rd, Coopers Plains, QLD 4108, Queensland, Australia.
Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Health and Food Science Precinct, Kessels Rd, Coopers Plains, QLD 4108, Queensland, Australia; ARC Industrial Transformation Training Centre for Uniquely Australian Foods, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Health and Food Science Precinct, Kessels Rd, Coopers Plains, QLD 4108, Queensland, Australia.
J Microbiol Methods. 2021 Mar;182:106169. doi: 10.1016/j.mimet.2021.106169. Epub 2021 Feb 15.
Different types of susceptibility tests are available to identify antimicrobial activity, including the disc agar diffusion and broth micro-dilution methods. In recent years, high throughput screening (HTS) methods have been considered and evaluated as an efficient method to rapidly monitor the antimicrobial potential of a wide range of plant products. The objective of this study was to test the ability of a 96-well plate reader as HTS method to evaluate the antimicrobial potential of extracts of Terminalia ferdinandiana (Kakadu plum). The main changes observed in the UV-VIS spectra of the bacteria samples were related to the biochemical and chemical compounds that might originate from the effect of the T. ferdinandiana extracts and the bacteria. Partial least squares discriminant analysis (PLS-DA) allowed the correct classification of samples according to the concentration of extract added to the culture (e.g. high, medium and low). The results of this study indicated that might be possible to record changes in the UV-VIS spectra associated with the interactions between bacteria and T. ferdinandiana extracts using a 96-well plate reader. The method was able to detect or differentiate between live and dead bacteria based on the UV-VIS spectra as a function of the addition of the T. ferdinandiana extracts.
有不同类型的药敏试验可用于鉴定抗菌活性，包括纸片琼脂扩散法和肉汤微量稀释法。近年来，高通量筛选（HTS）方法已被认为是一种有效的方法，可以快速监测广泛的植物产品的抗菌潜力。本研究的目的是测试 96 孔板读数器作为 HTS 方法的能力，以评估Terminalia ferdinandiana（卡卡杜李）提取物的抗菌潜力。细菌样品的紫外-可见光谱中观察到的主要变化与可能源于 T. ferdinandiana 提取物和细菌相互作用的生化和化学化合物有关。偏最小二乘判别分析（PLS-DA）允许根据添加到培养物中的提取物浓度（例如高、中、低）正确分类样品。本研究结果表明，使用 96 孔板读数器可能可以记录与细菌和 T. ferdinandiana 提取物相互作用相关的紫外-可见光谱变化。该方法能够根据添加 T. ferdinandiana 提取物后的紫外-可见光谱检测或区分活细菌和死细菌。
