CATP-8/P5A 型 ATP 酶调控 DMA-1 受体在树突分支中的内质网加工。

CATP-8/P5A ATPase Regulates ER Processing of the DMA-1 Receptor for Dendritic Branching.

机构信息

School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China; Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China; University of Chinese Academy of Sciences, Beijing 100049, China.

School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.

出版信息

Cell Rep. 2020 Sep 8;32(10):108101. doi: 10.1016/j.celrep.2020.108101.

DOI:10.1016/j.celrep.2020.108101

PMID:32905774

Abstract

Dendrite morphogenesis is essential for a neuron to establish its receptive field and is, thus, the anatomical basis for the proper functioning of the nervous system. The molecular mechanisms governing dendrite branching are not fully understood. Using the multi-dendritic PVD neuron in the nematode Caenorhabditis elegans, we identify CATP-8/P5A ATPase as a key regulator of dendrite branching that controls the translocation of the DMA-1 receptor to the endoplasmic reticulum (ER). The specific signal peptide of DMA-1 and the ATPase activity of CATP-8 are essential for this process. Our results reveal that P5A ATPase may regulate protein translocation in the ER.

摘要

树突形态发生对于神经元建立其感受野至关重要，因此是神经系统正常功能的解剖学基础。然而，控制树突分支的分子机制尚不完全清楚。利用线虫秀丽隐杆线虫中的多树突 PVD 神经元，我们鉴定出 CATP-8/P5A 型 ATP 酶作为树突分支的关键调节剂，控制 DMA-1 受体向内质网（ER）的易位。DMA-1 的特异信号肽和 CATP-8 的 ATP 酶活性对于这一过程是必需的。我们的结果揭示了 P5A ATP 酶可能调节 ER 中的蛋白易位。