Department of Biology, Faculty of Science, Mahasarakham University, Kantaravichai, Maha Sarakham Province, 44150, Thailand.
Medical Biotechnology, College of Medicine and Public Health, Flinders University, Bedford Park, SA, 5042, Australia.
Antonie Van Leeuwenhoek. 2021 Apr;114(4):365-377. doi: 10.1007/s10482-021-01519-5. Epub 2021 Feb 17.
An endophytic actinobacterium, strain PIP199, was isolated from a root sample of a native apricot growing on the Bedford Park campus of Flinders University, Adelaide, South Australia. The result of a polyphasic study showed that this strain was identified as a new member of the genus Amycolatopsis. Strain PIP199 is an aerobic actinobacterium with well-developed substrate mycelia and aerial mycelia that form short chains of spores. Amycolatopsis keratiniphila subsp. keratiniphila DSM 44409 (99.7%), Amycolatopsis lurida DSM 43134 (99.6%) and Amycolatopsis keratiniphila subsp. nogabecina DSM 44586 (99.4%) shared the highest 16S rRNA gene sequence similarity. A. keratiniphila subsp. keratiniphila DSM 44409 and A. lurida DSM 43134 were the closest phylogenetic neighbors. Chemotaxonomic data including major fatty acids, cell wall components and major menaquinones confirmed the affiliation of strain PIP199 to the genus Amycolatopsis. The phylogenetic analysis, physiological and biochemical studies and genomic study, allowed the genotypic and phenotypic differentiation of strain PIP199 and the closely related species with valid names. ANIb and dDDH values when compared to Amycolatopsis keratiniphila subsp. keratiniphila DSM 44409 were 87.3% and 36.4%, respectively. The name proposed for the new species is Amycolatopsis pittospori sp. nov. The type strain is PIP199 (= NRRL B-65536 = TBRC 10618).
从生长在南澳大利亚阿德莱德弗林德斯大学贝德福德公园校区的本地杏树根部分离到一株内生放线菌，菌株 PIP199。多相研究的结果表明，该菌株被鉴定为 Amycolatopsis 属的一个新成员。菌株 PIP199 是一种需氧放线菌，具有发育良好的基质菌丝和气生菌丝，形成短链孢子。Amycolatopsis keratiniphila subsp. keratiniphila DSM 44409（99.7%）、Amycolatopsis lurida DSM 43134（99.6%）和 Amycolatopsis keratiniphila subsp. nogabecina DSM 44586（99.4%）的 16S rRNA 基因序列相似性最高。Amycolatopsis keratiniphila subsp. keratiniphila DSM 44409 和 Amycolatopsis lurida DSM 43134 是最接近的系统发育近缘种。包括主要脂肪酸、细胞壁成分和主要menaquinones 的化学分类学数据证实了菌株 PIP199 属于 Amycolatopsis 属。系统发育分析、生理生化研究和基因组研究，使得菌株 PIP199 与具有有效名称的密切相关的种在基因型和表型上得以区分。与 Amycolatopsis keratiniphila subsp. keratiniphila DSM 44409 相比，ANIb 和 dDDH 值分别为 87.3%和 36.4%。新种的命名为 Amycolatopsis pittospori sp. nov.。模式菌株为 PIP199（=NRRL B-65536=TBRC 10618）。
